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Metabolic flux balance analysis and the in silico analysis of Escherichia coli K-12 gene deletions

k-12

Edwards JS, Palsson BO.
GSID: SYOhVtZZY7wJ
JS Edwards, BO Palsson - BMC bioinformatics, 2000 - bmcbioinformatics.biomedcentral …

Genome sequencing and bioinformatics are producing detailed lists of the molecular components contained in many prokaryotic organisms. From this 'parts catalogue' of a microbial cell, in silico representations of integrated metabolic functions can be constructed and analyzed …

Escherichia coli K-12 undergoes adaptive evolution to achieve in silico predicted optimal growth

k-12

Edwards JS, Palsson BO.
GSID: nVsDZZLJhqcJ
RU Ibarra, JS Edwards, BO Palsson - Nature, 2002 - nature.com

… coli K-12 on several substrates (acetate, succinate, malate, glucose and glycerol). All … coli K-12 on malate was predicted a priori by using PPP. The results for growth with malate … coli K-12 MG1655 annotated genome sequence and...

Genome-wide reconstruction of OxyR and SoxRS transcriptional regulatory networks under oxidative stress in Escherichia coli K-12 MG1655

k-12

Seo SW, Kim D, Palsson BO.
GSID: QrpPjS9jwy8J
SW Seo, D Kim, R Szubin, BO Palsson - Cell Reports, 2015 - Elsevier

Three transcription factors (TFs), OxyR, SoxR, and SoxS, play a critical role in transcriptional regulation of the defense system for oxidative stress in bacteria. However, their full genome-wide regulatory potential is unknown. Here, we perform a genome-scale reconstruction of...

Description and Interpretation of Adaptive Evolution of Escherichia coli K-12 MG1655 by Using a Genome-Scale In Silico Metabolic Model

k-12

Fong SS, Palsson BØ.
GSID: U5QGkTk9898J
SS Fong, JY Marciniak, BØ Palsson - Journal of bacteriology, 2003 - Am Soc Microbiol

… coli K-12 wild-type strain MG1655 was grown on 2 g of lactate per liter, we found that at 30C it operated in region 2 of the lactate PhPP (Fig. 2A and B), which is a region characterized by...

Adaptive Evolution of Escherichia coli K-12 MG1655 during Growth on a Nonnative Carbon Source, l-1,2-Propanediol

k-12

Lee DH, Palsson BØ.
GSID: FRPtvHCTUXIJ
DH Lee, BØ Palsson - Applied and environmental microbiology, 2010 - Am Soc Microbiol

… In this study, we evolved Escherichia coli K-12 MG1655 with a nonnative carbon source, L-1,2-propanediol (L-1,2-PDO), for 700 generations. We found that (i) experimental evolution of E. coli for 700 generations in 1,2-PDO-supplemented minimal medium resulted in …

Effective intercellular communication distances are determined by the relative time constants for cyto/chemokine secretion and diffusion

science instructors

Palsson BO.
GSID: B5ctToukfA0J
K Francis, BO Palsson - Proceedings of the National …, 1997 - National Acad Sciences

A cell's ability to effectively communicate with a neighboring cell is essential for tissue function and ultimately for the organism to which it belongs. One important mode of …

The developmental transcriptome of contrasting Arctic charr ( Salvelinus alpinus) morphs.

F1000Research

Gudbrandsson J, Ahi EP, Franzdottir SR, Kapralova KH, Kristjansson BK, Steinhaeuser SS, Maier VH, Johannesson IM, Snorrason SS, Jonsson ZO, Palsson A.
PMID: 27635217
F1000Res. 2015 Jun 01;4:136. doi: 10.12688/f1000research.6402.1. eCollection 2015.

Species and populations with parallel evolution of specific traits can help illuminate how predictable adaptations and divergence are at the molecular and developmental level. Following the last glacial period, dwarfism and specialized bottom feeding morphology evolved rapidly in several...

Understanding the Causes and Implications of Endothelial Metabolic Variation in Cardiovascular Disease through Genome-Scale Metabolic Modeling.

Frontiers in cardiovascular medicine

McGarrity S, Halldórsson H, Palsson S, Johansson PI, Rolfsson Ó.
PMID: 27148541
Front Cardiovasc Med. 2016 Apr 18;3:10. doi: 10.3389/fcvm.2016.00010. eCollection 2016.

High-throughput biochemical profiling has led to a requirement for advanced data interpretation techniques capable of integrating the analysis of gene, protein, and metabolic profiles to shed light on genotype-phenotype relationships. Herein, we consider the current state of knowledge of...

Coarse graining from variationally enhanced sampling applied to the Ginzburg-Landau model.

Proceedings of the National Academy of Sciences of the United States of America

Invernizzi M, Valsson O, Parrinello M.
PMID: 28292890
Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):3370-3374. doi: 10.1073/pnas.1618455114. Epub 2017 Mar 14.

A powerful way to deal with a complex system is to build a coarse-grained model capable of catching its main physical features, while being computationally affordable. Inevitably, such coarse-grained models introduce a set of phenomenological parameters, which are often...

Interactions of perfluoroalkyl substances with a phospholipid bilayer studied by neutron reflectometry.

Journal of colloid and interface science

Nouhi S, Ahrens L, Campos Pereira H, Hughes AV, Campana M, Gutfreund P, Palsson GK, Vorobiev A, Hellsing MS.
PMID: 29073553
J Colloid Interface Sci. 2018 Feb 01;511:474-481. doi: 10.1016/j.jcis.2017.09.102. Epub 2017 Sep 28.

The interactions between perfluoroalkyl substances (PFASs) and a phospholipid bilayer (1,2-dimyristoyl-sn-glycero-3-phosphocholine) were investigated at the molecular level using neutron reflectometry. Representative PFASs with different chain length and functional groups were selected in this study including: perfluorobutane sulfonate (PFBS), perfluorohexanoate...

Multi-radionuclide digital autoradiography of the intra-aortic atherosclerotic plaques using a monoclonal antibody targeting oxidized low-density lipoprotein.

American journal of nuclear medicine and molecular imaging

Orbom A, Jansson B, Schiopu A, Evans-Axelsson S, Nilsson J, Fredrikson GN, Strand SE.
PMID: 24753983
Am J Nucl Med Mol Imaging. 2014 Mar 20;4(2):172-80. eCollection 2014.

The aim of this study was to use multi-radionuclide autoradiography to compare the different distributions of three radiolabelled tracers in an atherosclerotic mouse model. This method, along with immunohistochemistry, was applied to investigate the intra-aortic distribution of 2-deoxy-2-[(18)F]fluoro-D-glucose ((18)F-FDG),...

Thermodynamical Description of a Quasi-First-Order Phase Transition from the Well-Tempered Ensemble.

Journal of chemical theory and computation

Valsson O, Parrinello M.
PMID: 26592265
J Chem Theory Comput. 2013 Dec 10;9(12):5267-76. doi: 10.1021/ct400859f. Epub 2013 Nov 21.

We explore the performance of the well-tempered ensemble combined with parallel tempering (PT-WTE) in obtaining a thermodynamical description of a given molecular system. We carefully explain the theoretical procedure employed to extract all the relevant thermodynamical quantities from a...

Showing 1 to 12 of 295 entries