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System wide cofactor turnovers can propagate metabolic stability between pathways.

Metabolic engineering communications

Yang Y, Guan YH, Villadsen J.
PMID: 29142824
Metab Eng Commun. 2016 Jun 16;3:196-204. doi: 10.1016/j.meteno.2016.06.002. eCollection 2016 Dec.

Metabolic homeostasis, or low-level metabolic steady state, has long been taken for granted in metabolic engineering, and research priority has always been given to understand metabolic flux control and regulation of the reaction network. In the past, this has...

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Yang Y, Guan YH, Villadsen J. System wide cofactor turnovers can propagate metabolic stability between pathways. Metab Eng Commun. 2016;3:196-204doi: 10.1016/j.meteno.2016.06.002.
Yang, Y., Guan, Y. H., & Villadsen, J. (2016). System wide cofactor turnovers can propagate metabolic stability between pathways. Metabolic engineering communications, 3196-204. https://doi.org/10.1016/j.meteno.2016.06.002
Yang, Y, et al. "System wide cofactor turnovers can propagate metabolic stability between pathways." Metabolic engineering communications vol. 3 (2016): 196-204. doi: https://doi.org/10.1016/j.meteno.2016.06.002
Yang Y, Guan YH, Villadsen J. System wide cofactor turnovers can propagate metabolic stability between pathways. Metab Eng Commun. 2016 Jun 16;3:196-204. doi: 10.1016/j.meteno.2016.06.002. eCollection 2016 Dec. PMID: 29142824; PMCID: PMC5678836.
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Characterization of a new hexasodium diphosphopentamolybdate hydrate, Na6[P2Mo5O23]x7H2O, by 23Na MQMAS NMR spectroscopy and X-ray powder diffraction.

Inorganic chemistry

Skibsted J, Brorson M, Villadsen J, Jakobsen HJ.
PMID: 11198870
Inorg Chem. 2000 Sep 04;39(18):4130-6. doi: 10.1021/ic000309j.

A novel hexasodium disphosphopentamolybdate hydrate, Na6[P2Mo5O23]x7H2O, has been identified using X-ray powder diffraction, 1H, 23Na, and 31P magic-angle spinning (MAS) NMR, and 23Na multiple-quantum (MQ) MAS NMR. Powder XRD reveals that the hydrate belongs to the triclinic spacegroup P1...

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Skibsted J, Brorson M, Villadsen J, et al. Characterization of a new hexasodium diphosphopentamolybdate hydrate, Na6[P2Mo5O23]x7H2O, by 23Na MQMAS NMR spectroscopy and X-ray powder diffraction. Inorg Chem. 2000;39(18):4130-6doi: 10.1021/ic000309j.
Skibsted, J., Brorson, M., Villadsen, J., & Jakobsen, H. J. (2000). Characterization of a new hexasodium diphosphopentamolybdate hydrate, Na6[P2Mo5O23]x7H2O, by 23Na MQMAS NMR spectroscopy and X-ray powder diffraction. Inorganic chemistry, 39(18), 4130-6. https://doi.org/10.1021/ic000309j
Skibsted, J, et al. "Characterization of a new hexasodium diphosphopentamolybdate hydrate, Na6[P2Mo5O23]x7H2O, by 23Na MQMAS NMR spectroscopy and X-ray powder diffraction." Inorganic chemistry vol. 39,18 (2000): 4130-6. doi: https://doi.org/10.1021/ic000309j
Skibsted J, Brorson M, Villadsen J, Jakobsen HJ. Characterization of a new hexasodium diphosphopentamolybdate hydrate, Na6[P2Mo5O23]x7H2O, by 23Na MQMAS NMR spectroscopy and X-ray powder diffraction. Inorg Chem. 2000 Sep 04;39(18):4130-6. doi: 10.1021/ic000309j. PMID: 11198870.
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Myths and realities of online clinical work.

Cyberpsychology & behavior : the impact of the Internet, multimedia and virtual reality on behavior and society

Fenichel M, Suler J, Barak A, Zelvin E, Jones G, Munro K, Meunier V, Walker-Schmucker W.
PMID: 12448785
Cyberpsychol Behav. 2002 Oct;5(5):481-97. doi: 10.1089/109493102761022904.

An international, multi-disciplinary group of online mental health professionals, under the auspices of the International Society for Mental Health Online, presents this 3rd-year report. This article highlights the experiences of ISMHO's Clinical Case Study Group members with regard to...

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Fenichel M, Suler J, Barak A, et al. Myths and realities of online clinical work. Cyberpsychol Behav. 2002;5(5):481-97doi: 10.1089/109493102761022904.
Fenichel, M., Suler, J., Barak, A., Zelvin, E., Jones, G., Munro, K., Meunier, V., & Walker-Schmucker, W. (2002). Myths and realities of online clinical work. Cyberpsychology & behavior : the impact of the Internet, multimedia and virtual reality on behavior and society, 5(5), 481-97. https://doi.org/10.1089/109493102761022904
Fenichel, Michael, et al. "Myths and realities of online clinical work." Cyberpsychology & behavior : the impact of the Internet, multimedia and virtual reality on behavior and society vol. 5,5 (2002): 481-97. doi: https://doi.org/10.1089/109493102761022904
Fenichel M, Suler J, Barak A, Zelvin E, Jones G, Munro K, Meunier V, Walker-Schmucker W. Myths and realities of online clinical work. Cyberpsychol Behav. 2002 Oct;5(5):481-97. doi: 10.1089/109493102761022904. PMID: 12448785.
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Modeling fermentations with recombinant microorganisms: formulation of a structured model.

Biotechnology and bioengineering

Nielsen J, Pedersen AG, Strudsholm K, Villadsen J.
PMID: 18600679
Biotechnol Bioeng. 1991 Apr 15;37(9):802-8. doi: 10.1002/bit.260370903.

A simple structured model is described and compared with experimental data for fermentations with recombinant. Escherichia coli. The model is a so-called compartment, model, where the different biomass components are lumped together in a few intracellular variables. The model...

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Nielsen J, Pedersen AG, Strudsholm K, et al. Modeling fermentations with recombinant microorganisms: formulation of a structured model. Biotechnol Bioeng. 1991;37(9):802-8doi: 10.1002/bit.260370903.
Nielsen, J., Pedersen, A. G., Strudsholm, K., & Villadsen, J. (1991). Modeling fermentations with recombinant microorganisms: formulation of a structured model. Biotechnology and bioengineering, 37(9), 802-8. https://doi.org/10.1002/bit.260370903
Nielsen, J, et al. "Modeling fermentations with recombinant microorganisms: formulation of a structured model." Biotechnology and bioengineering vol. 37,9 (1991): 802-8. doi: https://doi.org/10.1002/bit.260370903
Nielsen J, Pedersen AG, Strudsholm K, Villadsen J. Modeling fermentations with recombinant microorganisms: formulation of a structured model. Biotechnol Bioeng. 1991 Apr 15;37(9):802-8. doi: 10.1002/bit.260370903. PMID: 18600679.
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Degradation of pencillin-V in fermentation media.

Biotechnology and bioengineering

Christensen LH, Nielsen J, Villadsen J.
PMID: 18618681
Biotechnol Bioeng. 1994 Jun 20;44(2):165-9. doi: 10.1002/bit.260440204.

In Industrial production of penicillin there is a noticeable loss of the product through degradation reactions. It is shown that the degradation of penicillin-V, both in a complex and in a chemically defined medium, can be separated into a...

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Christensen LH, Nielsen J, Villadsen J. Degradation of pencillin-V in fermentation media. Biotechnol Bioeng. 1994;44(2):165-9doi: 10.1002/bit.260440204.
Christensen, L. H., Nielsen, J., & Villadsen, J. (1994). Degradation of pencillin-V in fermentation media. Biotechnology and bioengineering, 44(2), 165-9. https://doi.org/10.1002/bit.260440204
Christensen, L H, et al. "Degradation of pencillin-V in fermentation media." Biotechnology and bioengineering vol. 44,2 (1994): 165-9. doi: https://doi.org/10.1002/bit.260440204
Christensen LH, Nielsen J, Villadsen J. Degradation of pencillin-V in fermentation media. Biotechnol Bioeng. 1994 Jun 20;44(2):165-9. doi: 10.1002/bit.260440204. PMID: 18618681.
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Morphology and physiology of an alpha-amylase producing strain of Aspergillus oryzae during batch cultivations.

Biotechnology and bioengineering

Carlsen M, Spohr AB, Nielsen J, Villadsen J.
PMID: 18623577
Biotechnol Bioeng. 1996 Feb 05;49(3):266-76. doi: 10.1002/(SICI)1097-0290(19960205)49:3<266::AID-BIT4>3.0.CO;2-I.

The microscopic morphology, that is, total hyphal length and total number of tips, has been characterized during batch cultivations of Aspergillus oryzae. The specific growth rate estimated by measuring the total hyphal length (mu(h)) corresponds well with the specific...

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Carlsen M, Spohr AB, Nielsen J, et al. Morphology and physiology of an alpha-amylase producing strain of Aspergillus oryzae during batch cultivations. Biotechnol Bioeng. 1996;49(3):266-76doi: 10.1002/(SICI)1097-0290(19960205)49:3<266::AID-BIT4>3.0.CO;2-I.
Carlsen, M., Spohr, A. B., Nielsen, J., & Villadsen, J. (1996). Morphology and physiology of an alpha-amylase producing strain of Aspergillus oryzae during batch cultivations. Biotechnology and bioengineering, 49(3), 266-76. https://doi.org/10.1002/(SICI)1097-0290(19960205)49:3<266::AID-BIT4>3.0.CO;2-I
Carlsen, M, et al. "Morphology and physiology of an alpha-amylase producing strain of Aspergillus oryzae during batch cultivations." Biotechnology and bioengineering vol. 49,3 (1996): 266-76. doi: https://doi.org/10.1002/(SICI)1097-0290(19960205)49:3<266::AID-BIT4>3.0.CO;2-I
Carlsen M, Spohr AB, Nielsen J, Villadsen J. Morphology and physiology of an alpha-amylase producing strain of Aspergillus oryzae during batch cultivations. Biotechnol Bioeng. 1996 Feb 05;49(3):266-76. doi: 10.1002/(SICI)1097-0290(19960205)49:3<266::AID-BIT4>3.0.CO;2-I. PMID: 18623577.
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Transport of sugars via two anomer-specific sites on mannose-phosphotransferase system in Lactococcus cremoris: in vivo study of mechanism, kinetics, and adaptation.

Biotechnology and bioengineering

Benthin S, Nielsen J, Villadsen J.
PMID: 18613047
Biotechnol Bioeng. 1993 Aug 05;42(4):440-8. doi: 10.1002/bit.260420406.

Glucose uptake in Lactococcus lactis subsp. cremoris FD1 occurs via the mannose phosphotransferase system (Man-PTS), which is quite unspecific and allows transport of many different sugars and sugar analogues. It was previously shown (Benthin, S., Nielsen, J., Villadsen, J....

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Benthin S, Nielsen J, Villadsen J. Transport of sugars via two anomer-specific sites on mannose-phosphotransferase system in Lactococcus cremoris: in vivo study of mechanism, kinetics, and adaptation. Biotechnol Bioeng. 1993;42(4):440-8doi: 10.1002/bit.260420406.
Benthin, S., Nielsen, J., & Villadsen, J. (1993). Transport of sugars via two anomer-specific sites on mannose-phosphotransferase system in Lactococcus cremoris: in vivo study of mechanism, kinetics, and adaptation. Biotechnology and bioengineering, 42(4), 440-8. https://doi.org/10.1002/bit.260420406
Benthin, S, et al. "Transport of sugars via two anomer-specific sites on mannose-phosphotransferase system in Lactococcus cremoris: in vivo study of mechanism, kinetics, and adaptation." Biotechnology and bioengineering vol. 42,4 (1993): 440-8. doi: https://doi.org/10.1002/bit.260420406
Benthin S, Nielsen J, Villadsen J. Transport of sugars via two anomer-specific sites on mannose-phosphotransferase system in Lactococcus cremoris: in vivo study of mechanism, kinetics, and adaptation. Biotechnol Bioeng. 1993 Aug 05;42(4):440-8. doi: 10.1002/bit.260420406. PMID: 18613047.
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Structured modeling of a microbial system: I. A theoretical study of lactic acid fermentation.

Biotechnology and bioengineering

Nielsen J, Nikolajsen K, Villadsen J.
PMID: 18600692
Biotechnol Bioeng. 1991 Jun 05;38(1):1-10. doi: 10.1002/bit.260380102.

Most fermentation models presented in the literature are unstructured, i.e., the biomass composition is assumed constant during all operating conditions. These models are unable to simulate experiments carried out at widely different operating conditions. It is therefore interesting to...

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Nielsen J, Nikolajsen K, Villadsen J. Structured modeling of a microbial system: I. A theoretical study of lactic acid fermentation. Biotechnol Bioeng. 1991;38(1):1-10doi: 10.1002/bit.260380102.
Nielsen, J., Nikolajsen, K., & Villadsen, J. (1991). Structured modeling of a microbial system: I. A theoretical study of lactic acid fermentation. Biotechnology and bioengineering, 38(1), 1-10. https://doi.org/10.1002/bit.260380102
Nielsen, J, et al. "Structured modeling of a microbial system: I. A theoretical study of lactic acid fermentation." Biotechnology and bioengineering vol. 38,1 (1991): 1-10. doi: https://doi.org/10.1002/bit.260380102
Nielsen J, Nikolajsen K, Villadsen J. Structured modeling of a microbial system: I. A theoretical study of lactic acid fermentation. Biotechnol Bioeng. 1991 Jun 05;38(1):1-10. doi: 10.1002/bit.260380102. PMID: 18600692.
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Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations.

Biotechnology and bioengineering

Jørgensen H, Nielsen J, Villadsen J, Møllgaard H.
PMID: 18623271
Biotechnol Bioeng. 1995 Apr 20;46(2):117-31. doi: 10.1002/bit.260460205.

Based on a review of the Penicillium chrysogenum biochemistry a stoichiometric model has been set up. The model considers 61 internal fluxes and there are 49 intracellular metabolites which are assumed to be in pseudo-steady state. In addition to...

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Jørgensen H, Nielsen J, Villadsen J, et al. Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations. Biotechnol Bioeng. 1995;46(2):117-31doi: 10.1002/bit.260460205.
Jørgensen, H., Nielsen, J., Villadsen, J., & Møllgaard, H. (1995). Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations. Biotechnology and bioengineering, 46(2), 117-31. https://doi.org/10.1002/bit.260460205
Jørgensen, H, et al. "Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations." Biotechnology and bioengineering vol. 46,2 (1995): 117-31. doi: https://doi.org/10.1002/bit.260460205
Jørgensen H, Nielsen J, Villadsen J, Møllgaard H. Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations. Biotechnol Bioeng. 1995 Apr 20;46(2):117-31. doi: 10.1002/bit.260460205. PMID: 18623271.
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Who retires early and why? Determinants of early retirement pension among Danish employees 57-62 years.

European journal of ageing

Lund T, Villadsen E.
PMID: 28794742
Eur J Ageing. 2005 Nov 29;2(4):275-280. doi: 10.1007/s10433-005-0013-x. eCollection 2005 Dec.

The aim of this study was to identify demographic characteristics and occupational determinants of transition from employment to voluntary early retirement pension (ERP). A questionnaire-based survey among 365 employees in Denmark 57-62 years was performed in 2000, with a...

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Lund T, Villadsen E. Who retires early and why? Determinants of early retirement pension among Danish employees 57-62 years. Eur J Ageing. 2005;2(4):275-280doi: 10.1007/s10433-005-0013-x.
Lund, T., & Villadsen, E. (2005). Who retires early and why? Determinants of early retirement pension among Danish employees 57-62 years. European journal of ageing, 2(4), 275-280. https://doi.org/10.1007/s10433-005-0013-x
Lund, Thomas, and Villadsen, Ebbe. "Who retires early and why? Determinants of early retirement pension among Danish employees 57-62 years." European journal of ageing vol. 2,4 (2005): 275-280. doi: https://doi.org/10.1007/s10433-005-0013-x
Lund T, Villadsen E. Who retires early and why? Determinants of early retirement pension among Danish employees 57-62 years. Eur J Ageing. 2005 Nov 29;2(4):275-280. doi: 10.1007/s10433-005-0013-x. eCollection 2005 Dec. PMID: 28794742; PMCID: PMC5546287.
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Osmotic stress changes carbohydrate partitioning and fructose-2,6-bisphosphate metabolism in barley leaves.

Functional plant biology : FPB

Villadsen D, Rung JH, Nielsen TH.
PMID: 32689199
Funct Plant Biol. 2005 Nov;32(11):1033-1043. doi: 10.1071/FP05102.

Carbohydrate metabolism was investigated in barley leaves subjected to drought or osmotic stress induced by sorbitol incubation. Both drought and osmotic stress resulted in accumulation of hexoses, depletion of sucrose and starch, and 5-10-fold increase in the level of...

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Villadsen D, Rung JH, Nielsen TH. Osmotic stress changes carbohydrate partitioning and fructose-2,6-bisphosphate metabolism in barley leaves. Funct Plant Biol. 2005;32(11):1033-1043doi: 10.1071/FP05102.
Villadsen, D., Rung, J. H., & Nielsen, T. H. (2005). Osmotic stress changes carbohydrate partitioning and fructose-2,6-bisphosphate metabolism in barley leaves. Functional plant biology : FPB, 32(11), 1033-1043. https://doi.org/10.1071/FP05102
Villadsen, Dorthe, et al. "Osmotic stress changes carbohydrate partitioning and fructose-2,6-bisphosphate metabolism in barley leaves." Functional plant biology : FPB vol. 32,11 (2005): 1033-1043. doi: https://doi.org/10.1071/FP05102
Villadsen D, Rung JH, Nielsen TH. Osmotic stress changes carbohydrate partitioning and fructose-2,6-bisphosphate metabolism in barley leaves. Funct Plant Biol. 2005 Nov;32(11):1033-1043. doi: 10.1071/FP05102. PMID: 32689199.
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Comparative transcriptomics of ice-crawlers demonstrates cold specialization constrains niche evolution in a relict lineage.

Evolutionary applications

Schoville SD, Simon S, Bai M, Beethem Z, Dudko RY, Eberhard MJB, Frandsen PB, Küpper SC, Machida R, Verheij M, Willadsen PC, Zhou X, Wipfler B.
PMID: 33664782
Evol Appl. 2020 Sep 11;14(2):360-382. doi: 10.1111/eva.13120. eCollection 2021 Feb.

Key changes in ecological niche space are often critical to understanding how lineages diversify during adaptive radiations. However, the converse, or understanding why some lineages are depauperate and relictual, is more challenging, as many factors may constrain niche evolution....

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Schoville SD, Simon S, Bai M, et al. Comparative transcriptomics of ice-crawlers demonstrates cold specialization constrains niche evolution in a relict lineage. Evol Appl. 2020;14(2):360-382doi: 10.1111/eva.13120.
Schoville, S. D., Simon, S., Bai, M., Beethem, Z., Dudko, R. Y., Eberhard, M. J. B., Frandsen, P. B., Küpper, S. C., Machida, R., Verheij, M., Willadsen, P. C., Zhou, X., & Wipfler, B. (2021). Comparative transcriptomics of ice-crawlers demonstrates cold specialization constrains niche evolution in a relict lineage. Evolutionary applications, 14(2), 360-382. https://doi.org/10.1111/eva.13120
Schoville, Sean D, et al. "Comparative transcriptomics of ice-crawlers demonstrates cold specialization constrains niche evolution in a relict lineage." Evolutionary applications vol. 14,2 (2021): 360-382. doi: https://doi.org/10.1111/eva.13120
Schoville SD, Simon S, Bai M, Beethem Z, Dudko RY, Eberhard MJB, Frandsen PB, Küpper SC, Machida R, Verheij M, Willadsen PC, Zhou X, Wipfler B. Comparative transcriptomics of ice-crawlers demonstrates cold specialization constrains niche evolution in a relict lineage. Evol Appl. 2020 Sep 11;14(2):360-382. doi: 10.1111/eva.13120. eCollection 2021 Feb. PMID: 33664782; PMCID: PMC7896716.
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