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Showing 1 to 12 of 27 entries
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Influenza Vaccine With Consensus Internal Antigens as Immunogens Provides Cross-Group Protection Against Influenza A Viruses.

Frontiers in microbiology

Xie X, Zhao C, He Q, Qiu T, Yuan S, Ding L, Liu L, Jiang L, Wang J, Zhang L, Zhang C, Wang X, Zhou D, Zhang X, Xu J.
PMID: 31379782
Front Microbiol. 2019 Jul 16;10:1630. doi: 10.3389/fmicb.2019.01630. eCollection 2019.

Given that continuing antigenic shift and drift of influenza A viruses result in the escape from previous vaccine-induced immune protection, a universal influenza vaccine has been actively sought. However, there were very few vaccines capable of eliciting cross-group ant-influenza...

Human Growth and Growth Hormone: From Antiquity to the Recominant Age to the Future.

Frontiers in endocrinology

Graber E, Reiter EO, Rogol AD.
PMID: 34290673
Front Endocrinol (Lausanne). 2021 Jul 05;12:709936. doi: 10.3389/fendo.2021.709936. eCollection 2021.

Since antiquity Man has been fascinated by the variations in human (and animal) growth. Stories and art abound about giants and little people. Modern genetics have solved some of etiologies at both extremes of growth. Serious study began with...

Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.

Cell & bioscience

Ke F, Yu XD, Wang ZH, Gui JF, Zhang QY.
PMID: 34991685
Cell Biosci. 2022 Jan 06;12(1):6. doi: 10.1186/s13578-021-00742-x.

BACKGROUND: Ranaviruses (family Iridoviridae) are promiscuous pathogens that can infect across species barriers in poikilotherms and can replicate in amphibian and fish cells and even in cultured mammalian cells. However, as nucleocytoplasmic large DNA viruses (NCLDVs), their replication and...

Insulin discovery: A pivotal point in medical history.

Metabolism: clinical and experimental

Falcetta P, Aragona M, Bertolotto A, Bianchi C, Campi F, Garofolo M, Del Prato S.
PMID: 34838778
Metabolism. 2021 Nov 25;127:154941. doi: 10.1016/j.metabol.2021.154941. Epub 2021 Nov 25.

The discovery of insulin in 1921 - due to the efforts of the Canadian research team based in Toronto - has been a landmark achievement in the history of medicine. Lives of people with diabetes were changed forever, considering...

[Transfer and Expression of Recombinant Human Thrombopoietin Gene in COS-7 Cells and Mice In Vivo].

Zhongguo shi yan xue ye xue za zhi

Zang WP, Wei XD, Fu S, Wang SW, Tang J, Wang DB.
PMID: 12578637
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2001 Mar;9(1):14-17.

In order to investigate the expression of recombinant TPO gene in COS-7 cells and in vivo of the mouse model, eukaryotic expressing plasmid pcd2/TPO with human TPO cDNA was constructed with DNA recombinant techniques. The plasmid pcd2/TPO was transiently...

pELMO, an optimised in-house cloning vector.

AMB Express

Ramos AE, Muñoz M, Moreno-Pérez DA, Patarroyo MA.
PMID: 28116699
AMB Express. 2017 Dec;7(1):26. doi: 10.1186/s13568-017-0324-2. Epub 2017 Jan 24.

DNA cloning is an essential tool regarding DNA recombinant technology as it allows the replication of foreign DNA fragments within a cell. pELMO was here constructed as an in-house cloning vector for rapid and low-cost PCR product propagation; it...

Simultaneous Detection of CDC Category "A" DNA and RNA Bioterrorism Agents by Use of Multiplex PCR & RT-PCR Enzyme Hybridization Assays.

Viruses

He J, Kraft AJ, Fan J, Van Dyke M, Wang L, Bose ME, Khanna M, Metallo JA, Henrickson KJ.
PMID: 20224751
Viruses. 2009;1(3):441-459. doi: 10.3390/v1030441.

Assays to simultaneously detect multiple potential agents of bioterrorism are limited. Two multiplex PCR and RT-PCR enzyme hybridization assays (mPCR-EHA, mRT-PCR-EHA) were developed to simultaneously detect many of the CDC category "A" bioterrorism agents. The "Bio T" DNA assay...

Recombinant MVA-prime elicits neutralizing antibody responses by inducing antigen-specific B cells in the germinal center.

NPJ vaccines

Eslamizar L, Petrovas C, Leggat DJ, Furr K, Lifton ML, Levine G, Ma S, Fletez-Brant C, Hoyland W, Prabhakaran M, Narpala S, Boswell K, Yamamoto T, Liao HX, Pickup D, Ramsburg E, Sutherland L, McDermott A, Roederer M, Montefiori D, Koup RA, Haynes BF, Letvin NL, Santra S.
PMID: 33495459
NPJ Vaccines. 2021 Jan 25;6(1):15. doi: 10.1038/s41541-020-00277-1.

The RV144 HIV-1 vaccine trial has been the only clinical trial to date that has shown any degree of efficacy and associated with the presence of vaccine-elicited HIV-1 envelope-specific binding antibody and CD4+ T-cell responses. This trial also showed...

Insulin discovery: A pivotal point in medical history.

Metabolism: clinical and experimental

Falcetta P, Aragona M, Bertolotto A, Bianchi C, Campi F, Garofolo M, Del Prato S.
PMID: 34838778
Metabolism. 2021 Nov 25;127:154941. doi: 10.1016/j.metabol.2021.154941. Epub 2021 Nov 25.

The discovery of insulin in 1921 - due to the efforts of the Canadian research team based in Toronto - has been a landmark achievement in the history of medicine. Lives of people with diabetes were changed forever, considering...

Insulin discovery: A pivotal point in medical history.

Metabolism: clinical and experimental

Falcetta P, Aragona M, Bertolotto A, Bianchi C, Campi F, Garofolo M, Del Prato S.
PMID: 34838778
Metabolism. 2021 Nov 25;127:154941. doi: 10.1016/j.metabol.2021.154941. Epub 2021 Nov 25.

The discovery of insulin in 1921 - due to the efforts of the Canadian research team based in Toronto - has been a landmark achievement in the history of medicine. Lives of people with diabetes were changed forever, considering...

Adding Molecules to Food, Pros and Cons: A Review on Synthetic and Natural Food Additives.

Comprehensive reviews in food science and food safety

Carocho M, Barreiro MF, Morales P, Ferreira ICFR.
PMID: 33412697
Compr Rev Food Sci Food Saf. 2014 Jul;13(4):377-399. doi: 10.1111/1541-4337.12065.

The pressing issue to feed the increasing world population has created a demand to enhance food production, which has to be cheaper, but at the same time must meet high quality standards. Taste, appearance, texture, and microbiological safety are...

Cloning and Expression of the Gene Coding for the Fusion Protein rhGM-CSF/LIF.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Zhang XF, Ge ZL, Sun LY, Ding QM, Zhang QW, Cao H, Yuan LZ.
PMID: 12215780
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1997;29(5):425-431.

A fusion gene was constructed in a plasmid in which the coding regions of human GM_CSF and LIF cDNAs were connected by a synthetic linker sequence encoding a short peptide G-S-G-G-S through DNA recombinant techniques. It was then subcloned...

Showing 1 to 12 of 27 entries