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Showing 1 to 12 of 932 entries
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In vivo bioluminescence imaging for viable human neural stem cells incorporated within in situ gelatin hydrogels.

EJNMMI research

Hwang DW, Park KM, Shim HK, Jin Y, Oh HJ, Oh SW, Lee S, Youn H, Joung YK, Lee HJ, Kim SU, Park KD, Lee DS.
PMID: 26116122
EJNMMI Res. 2014 Dec;4(1):61. doi: 10.1186/s13550-014-0061-3. Epub 2014 Nov 12.

BACKGROUND: Three-dimensional (3D) hydrogel-based stem cell therapies contribute to enhanced therapeutic efficacy in treating diseases, and determining the optimal mechanical strength of the hydrogel in vivo is important for therapeutic success. We evaluated the proliferation of human neural stem...

Detection of rabbit IgG by using functional magnetic particles and an enzyme-conjugated antibody with a homemade magnetic microplate.

Chemistry Central journal

Tsai H, Lu YH, Liao HX, Wu SW, Yu FY, Fuh CB.
PMID: 25745512
Chem Cent J. 2015 Feb 22;9:8. doi: 10.1186/s13065-015-0088-1. eCollection 2015.

BACKGROUND: The enzyme-linked immunosorbent assay (ELISA) has been used for diagnosing medical and plant pathologies. In addition, it is used for quality-control evaluations in various industries. The ELISA is the simplest method for obtaining excellent results; however, it is...

Horseradish peroxidase and aptamer dual-functionalized nanoprobe for the amplification detection of alpha-methylacyl-CoA racemase.

Analytica chimica acta

Zeng Y, Zheng A, Wu J, Cai Z, Huang A, Liu X.
PMID: 26547498
Anal Chim Acta. 2015 Oct 29;899:100-5. doi: 10.1016/j.aca.2015.10.007. Epub 2015 Oct 20.

Alpha-methylacyl-CoA racemase (AMACR) is over-expressed in many cancer types and can serve as a novel diagnostic biomarker. Development of convenient and sensitive detection methods of AMACR is of particular importance for cancer diagnosis. Aptamers are a type of recognition...

Somatotopy in the Medullary Dorsal Horn As a Basis for Orofacial Reflex Behavior.

Frontiers in neurology

Panneton WM, Pan B, Gan Q.
PMID: 29066998
Front Neurol. 2017 Oct 10;8:522. doi: 10.3389/fneur.2017.00522. eCollection 2017.

The somatotopy of the trigeminocervical complex of the rat was defined as a basis for describing circuitry for reflex behaviors directed through the facial motor nucleus. Thus, transganglionic transport of horseradish peroxidase conjugates applied to individual nerves/peripheral receptive fields...

High throughput flow injection bioluminometric method for olive oil antioxidant capacity.

Food chemistry

Minioti KS, Georgiou CA.
PMID: 26003372
Food Chem. 2008 Jul 15;109(2):455-61. doi: 10.1016/j.foodchem.2007.12.077. Epub 2008 Jan 12.

This paper describes a rapid flow injection automated method for the determination of olive oil total antioxidant capacity. The chemistry involved is the horseradish peroxidase (HRP) catalysed oxidation of luminol by hydrogen peroxide. Oxidation results in light emission (bioluminescence)...

Fluorescence in situ hybridization and sequential catalyzed reporter deposition (2C-FISH) for the flow cytometric sorting of freshwater ultramicrobacteria.

Frontiers in microbiology

Neuenschwander SM, Salcher MM, Pernthaler J.
PMID: 25873914
Front Microbiol. 2015 Mar 31;6:247. doi: 10.3389/fmicb.2015.00247. eCollection 2015.

Flow cytometric sorting is a powerful tool to physically separate cells within mixed microbial communities. If combined with phylogenetic staining (fluorescence in situ hybridization, FISH) it allows to specifically sort defined genotypic microbial populations from complex natural samples. However,...

Lignin synthesis: The generation of hydrogen peroxide and superoxide by horseradish peroxidase and its stimulation by manganese (II) and phenols.

Planta

Halliwell B.
PMID: 24414365
Planta. 1978 Jan;140(1):81-8. doi: 10.1007/BF00389384.

The enzyme horseradish peroxidase (EC 1.11.1.7) catalyses oxidation of NADH. NADH oxidation is prevented by addition of the enzyme superoxide dismutase (EC 1.15.1.1) to the reaction mixture before adding peroxidase but addition of dismutase after peroxidase has little inhibitory...

Receptor-mediated uptake of labeled transferrin by embryonic chicken dorsal root ganglion neurons in culture.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience

Markelonis GJ, Oh TH, Park LP, Azari P, Max SR.
PMID: 24874753
Int J Dev Neurosci. 1985;3(3):257-66. doi: 10.1016/0736-5748(85)90030-9.

Transferrin is a growth-promoting plasma protein which is known to occur within developing neurons. Since little information exists on the process by which transferrin is internalized by neurons, we studied this process using dissociated embryonic chicken dorsal root ganglion...

Naked-Eye Coulometric Sensor Using a Longitudinally Oriented Ag Band Electrode in a Microfluidic Channel.

Analytical chemistry

Oh JM, Chow KF.
PMID: 27064358
Anal Chem. 2016 May 03;88(9):4849-56. doi: 10.1021/acs.analchem.6b00552. Epub 2016 Apr 11.

In this Article, we report a coulometric sensing platform that is capable of sensing analytes on a working electrode and providing a visual readout of the analyte concentration on a silver (Ag) band counter electrode in a microchannel. The...

High-Throughput Production of Chromium(III) Complexes for Antibody Immobilization.

Analytical chemistry

Welch NG, Scoble JA, Easton CD, Williams CC, Bradford BJ, Mamedova LK, Pigram PJ, Muir BW.
PMID: 27644116
Anal Chem. 2016 Oct 18;88(20):10102-10110. doi: 10.1021/acs.analchem.6b02531. Epub 2016 Oct 05.

A robot-assisted high-throughput methodology was employed to produce chromium(III) complexes suitable for the surface modification of the commercially available PerkinElmer Optiplate96 well plate for use in enzyme-linked immunosorbent assays (ELISAs). The complexes were immobilized to the native functionality of...

Screen Printed Carbon Electrode Based Electrochemical Immunosensor for the Detection of Dengue NS1 Antigen.

Diagnostics (Basel, Switzerland)

Parkash O, Yean CY, Shueb RH.
PMID: 26852684
Diagnostics (Basel). 2014 Nov 20;4(4):165-80. doi: 10.3390/diagnostics4040165.

An electrochemical immunosensor modified with the streptavidin/biotin system on screen printed carbon electrodes (SPCEs) for the detection of the dengue NS1 antigen was developed in this study. Monoclonal anti-NS1 capture antibody was immobilized on streptavidin-modified SPCEs to increase the...

Functioning of Microsomal Cytochrome P450s: Murburn Concept Explains the Metabolism of Xenobiotics in Hepatocytes.

Frontiers in pharmacology

Manoj KM, Parashar A, Gade SK, Venkatachalam A.
PMID: 27445805
Front Pharmacol. 2016 Jun 23;7:161. doi: 10.3389/fphar.2016.00161. eCollection 2016.

Using oxygen and NADPH, the redox enzymes cytochrome P450 (CYP) and its reductase (CPR) work in tandem to carry out the phase I metabolism of a vast majority of drugs and xenobiotics. As per the erstwhile understanding of the...

Showing 1 to 12 of 932 entries