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Showing 1 to 12 of 31 entries
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Isolation, Chemical Fingerprinting and Simultaneous Quantification of Four Compounds from Tanacetum gracile Using a Validated HPLC-ESI-QTOF-Mass Spectrometry Method.

Journal of chromatographic science

Sharma N, Kumar C, Dutt P, Gupta S, Satti NK, Chandra S, Kitchlu S, Paul S, Vishwakarma RA, Verma MK.
PMID: 26951542
J Chromatogr Sci. 2016 May-Jun;54(5):796-804. doi: 10.1093/chromsci/bmw013. Epub 2016 Mar 06.

The present study was conducted to carry out the phytochemical investigation of Tanacetum gracile Hook. f. & Thomson and to develop a method for the simultaneous quantification of the isolated compounds in the extracts ofT. gracile growing in different...

A direct sensitivity comparison between flow-modulated comprehensive 2D and 1D GC in untargeted and targeted MS-based experiments.

Journal of separation science

Tranchida PQ, Franchina FA, Zoccali M, Bonaccorsi I, Cacciola F, Mondello L.
PMID: 23868497
J Sep Sci. 2013 Sep;36(17):2746-52. doi: 10.1002/jssc.201300423. Epub 2013 Jul 19.

The present contribution is focused on the measurement of the analytical sensitivity attained in untargeted/targeted MS/MS experiments, performed using flow-modulator comprehensive 2D and 1D GC. The comprehensive 2D experiment was performed by diverting part of the high flow (circa...

A home-brew real-time PCR assay for reliable detection and quantification of mature miR-122.

Applied immunohistochemistry & molecular morphology : AIMM

Naderi M, Abdul Tehrani H, Soleimani M, Shabani I, Hashemi SM.
PMID: 25075470
Appl Immunohistochem Mol Morphol. 2015 Sep;23(8):601-6. doi: 10.1097/PAI.0000000000000125.

miR-122 is a liver-specific miRNA that has significant gene expression alterations in response to specific pathophysiological circumstances of liver such as drug-induced liver injury, hepatocellular carcinoma, and hepatitis B and C virus infections. Therefore, accurate and precise quantification of...

Highly multiplexed single-cell quantitative PCR.

PloS one

VanInsberghe M, Zahn H, White AK, Petriv OI, Hansen CL.
PMID: 29377915
PLoS One. 2018 Jan 29;13(1):e0191601. doi: 10.1371/journal.pone.0191601. eCollection 2018.

We present a microfluidic device for rapid gene expression profiling in single cells using multiplexed quantitative polymerase chain reaction (qPCR). This device integrates all processing steps, including cell isolation and lysis, complementary DNA synthesis, pre-amplification, sample splitting, and measurement...

Recent advances in single-molecule detection on micro- and nano-fluidic devices.

Electrophoresis

Liu C, Qu Y, Luo Y, Fang N.
PMID: 22134976
Electrophoresis. 2011 Nov;32(23):3308-18. doi: 10.1002/elps.201100159.

Single-molecule detection (SMD) allows static and dynamic heterogeneities from seemingly equal molecules to be revealed in the studies of molecular structures and intra- and inter-molecular interactions. Micro- and nanometer-sized structures, including channels, chambers, droplets, etc., in microfluidic and nanofluidic...

Overview of strategies for addressing BRIs in drug discovery: Impact on optimization and design.

Chemico-biological interactions

Humphreys WG.
PMID: 21238435
Chem Biol Interact. 2011 Jun 30;192(1):56-9. doi: 10.1016/j.cbi.2011.01.005. Epub 2011 Jan 13.

The sensitive and specific detection of adducts derived from reactive intermediates during discovery metabolite profiling has been made feasible by advances in LC-MS/MS instrumentation. Many companies employ screens with nucleophilic trapping agents as a routine part of early screening...

A study of the precision and accuracy of peak quantification in comprehensive two-dimensional liquid chromatography in time.

Journal of chromatography. A

Thekkudan DF, Rutan SC, Carr PW.
PMID: 20462587
J Chromatogr A. 2010 Jun 25;1217(26):4313-27. doi: 10.1016/j.chroma.2010.04.039. Epub 2010 Apr 21.

Simulated chromatographic data were used to determine the precision and accuracy in the estimation of peak volumes (i.e., peak sizes) in comprehensive two-dimensional liquid chromatography in time (LCxLC). Peak volumes were determined both by summing the areas in the...

Increasing hybridization rate and sensitivity of bead-based assays using isotachophoresis.

Angewandte Chemie (International ed. in English)

Shintaku H, Palko JW, Sanders GM, Santiago JG.
PMID: 25303671
Angew Chem Int Ed Engl. 2014 Dec 08;53(50):13813-6. doi: 10.1002/anie.201408403. Epub 2014 Oct 10.

We present an electrokinetic technique to increase the reaction rate and sensitivity of bead-based assays. We use isotachophoresis (ITP) to preconcentrate and co-focus target molecules and beads into a single ITP zone. The process achieves rapid mixing, stirring, and...

Parts per Million Powder X-ray Diffraction.

Analytical chemistry

Newman JA, Schmitt PD, Toth SJ, Deng F, Zhang S, Simpson GJ.
PMID: 26465382
Anal Chem. 2015 Nov 03;87(21):10950-5. doi: 10.1021/acs.analchem.5b02758. Epub 2015 Oct 22.

Here we demonstrate the use of second harmonic generation (SHG) microscopy-guided synchrotron powder X-ray diffraction (PXRD) for the detection of trace crystalline active pharmaceutical ingredients in a common polymer blend. The combined instrument is capable of detecting 100 ppm...

Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping.

Lab on a chip

Haber JM, Gascoyne PRC, Sokolov K.
PMID: 28703830
Lab Chip. 2017 Aug 08;17(16):2821-2830. doi: 10.1039/c7lc00211d.

Rapid detection and characterization of pathogens in patients with bloodstream infections (BSIs) is a persistent problem for modern medicine, as current techniques are slow or provide incomplete diagnostic information. Real-time polymerase chain reaction (qPCR) allows specific detection of a...

Shifting the Linear Range in Electrospray Ionization by In-Source Collision-Induced Dissociation.

Chemical & pharmaceutical bulletin

Ishii H, Yamaguchi H, Mano N.
PMID: 27039832
Chem Pharm Bull (Tokyo). 2016;64(4):356-9. doi: 10.1248/cpb.c15-00741.

The goal of this study was to demonstrate the utility of in-source collision-induced dissociation for shifting the linear range in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). The linear ranges for uracil, deoxyuridine, and uridine were shifted from 0.3-300, 1-100,...

Evaluating single molecule detection methods for microarrays with high dynamic range for quantitative single cell analysis.

Scientific reports

Salehi-Reyhani A.
PMID: 29263350
Sci Rep. 2017 Dec 20;7(1):17957. doi: 10.1038/s41598-017-18303-z.

Single molecule microarrays have been used in quantitative proteomics, in particular, single cell analysis requiring high sensitivity and ultra-low limits of detection. In this paper, several image analysis methods are evaluated for their ability to accurately enumerate single molecules...

Showing 1 to 12 of 31 entries