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Haber JM, Gascoyne PRC, Sokolov K. Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping. Lab Chip. 2017;17(16):2821-2830doi: 10.1039/c7lc00211d.
Haber, J. M., Gascoyne, P. R. C., & Sokolov, K. (2017). Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping. Lab on a chip, 17(16), 2821-2830. https://doi.org/10.1039/c7lc00211d
Haber, J M, et al. "Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping." Lab on a chip vol. 17,16 (2017): 2821-2830. doi: https://doi.org/10.1039/c7lc00211d
Haber JM, Gascoyne PRC, Sokolov K. Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping. Lab Chip. 2017 Aug 08;17(16):2821-2830. doi: 10.1039/c7lc00211d. PMID: 28703830; PMCID: PMC5612715.
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Lab Chip. 2017 Aug 08;17(16):2821-2830. doi: 10.1039/c7lc00211d.

Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping.

Lab on a chip

J M Haber, P R C Gascoyne, K Sokolov

Affiliations

  1. Department of Biomedical Engineering, University of Texas at Austin, Austin, TX 78712, USA.

PMID: 28703830 PMCID: PMC5612715 DOI: 10.1039/c7lc00211d

Abstract

Rapid detection and characterization of pathogens in patients with bloodstream infections (BSIs) is a persistent problem for modern medicine, as current techniques are slow or provide incomplete diagnostic information. Real-time polymerase chain reaction (qPCR) allows specific detection of a wide range of targets and quantification of pathogenic burdens to aid in treatment planning. However, new technological advances are required for a rapid and multiplex implementation of qPCR in clinical applications. In this paper, the feasibility of a novel microfluidic platform for qPCR is presented, integrating highly sensitive, label-free localized surface plasmon resonance (LSPR) imaging of DNA hybridization into a recirculating chip design for real-time analysis. Single target and multiplex detection of DNA target amplification are demonstrated, with a limit of detection of 5 fg μL

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