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Sharma N, Kumar C, Dutt P, et al. Isolation, Chemical Fingerprinting and Simultaneous Quantification of Four Compounds from Tanacetum gracile Using a Validated HPLC-ESI-QTOF-Mass Spectrometry Method. J Chromatogr Sci. 2016;54(5):796-804doi: 10.1093/chromsci/bmw013.
Sharma, N., Kumar, C., Dutt, P., Gupta, S., Satti, N. K., Chandra, S., Kitchlu, S., Paul, S., Vishwakarma, R. A., & Verma, M. K. (2016). Isolation, Chemical Fingerprinting and Simultaneous Quantification of Four Compounds from Tanacetum gracile Using a Validated HPLC-ESI-QTOF-Mass Spectrometry Method. Journal of chromatographic science, 54(5), 796-804. https://doi.org/10.1093/chromsci/bmw013
Sharma, Neha, et al. "Isolation, Chemical Fingerprinting and Simultaneous Quantification of Four Compounds from Tanacetum gracile Using a Validated HPLC-ESI-QTOF-Mass Spectrometry Method." Journal of chromatographic science vol. 54,5 (2016): 796-804. doi: https://doi.org/10.1093/chromsci/bmw013
Sharma N, Kumar C, Dutt P, Gupta S, Satti NK, Chandra S, Kitchlu S, Paul S, Vishwakarma RA, Verma MK. Isolation, Chemical Fingerprinting and Simultaneous Quantification of Four Compounds from Tanacetum gracile Using a Validated HPLC-ESI-QTOF-Mass Spectrometry Method. J Chromatogr Sci. 2016 May-Jun;54(5):796-804. doi: 10.1093/chromsci/bmw013. Epub 2016 Mar 06. PMID: 26951542; PMCID: PMC4890456.
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J Chromatogr Sci. 2016 May-Jun;54(5):796-804. doi: 10.1093/chromsci/bmw013. Epub 2016 Mar 06.

Isolation, Chemical Fingerprinting and Simultaneous Quantification of Four Compounds from Tanacetum gracile Using a Validated HPLC-ESI-QTOF-Mass Spectrometry Method.

Journal of chromatographic science

Neha Sharma, Chetan Kumar, Prabhu Dutt, Suphla Gupta, Naresh K Satti, Suresh Chandra, Surinder Kitchlu, Satya Paul, Ram A Vishwakarma, Mahendra K Verma

Affiliations

  1. Analytical Chemistry Division (Instrumentation), CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
  2. Natural Product Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
  3. Plant Biotechnology Department, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
  4. Genetic Resource & Agrotech. Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
  5. Department of Chemistry, University of Jammu, Jammu 180006, India.
  6. Analytical Chemistry Division (Instrumentation), CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India [email protected] [email protected].

PMID: 26951542 PMCID: PMC4890456 DOI: 10.1093/chromsci/bmw013

Abstract

The present study was conducted to carry out the phytochemical investigation of Tanacetum gracile Hook. f. & Thomson and to develop a method for the simultaneous quantification of the isolated compounds in the extracts ofT. gracile growing in different locations. Cluster analysis rectangular similarity matrix was performed to understand the chemical fingerprinting variations in the extracts. High-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS) was used to quantify four bioactive compounds, and separation of the compounds was achieved on a reverse-phase C8 column using a mobile phase of acetonitrile: 0.1% formic acid in water with a gradient elution by maintaining the flow rate of 300 μL/min. The QTOF-MS was operated using the electro-spray ionization technique with the positive ion polarity mode. The calibration curves of four marker compounds were linear over the concentration range of 3.12-100 ng/µL (R(2)> 0.996). A specific, accurate and precise HPLC-ESI-QTOF-MS method was optimized for the determination of kaempferol, ketoplenolide, tetramethoxyflavone and artemetin both individually and simultaneously. Quantification of these chemical markers in different extracts was carried out using this validated method. Kaempferol was isolated for the first time from T. gracile.

© The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected].

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