Display options
Cite
Yuan CC, Huang HC, Tsai LC, et al. Cytokeratin-19 associated with apoptosis and chemosensitivity in human cervical cancer cells. Apoptosis. 1997;2(1):101-5doi: 10.1023/a:1026448027870.
Yuan, C. C., Huang, H. C., Tsai, L. C., Ng, H. T., & Huang, T. S. (1997). Cytokeratin-19 associated with apoptosis and chemosensitivity in human cervical cancer cells. Apoptosis : an international journal on programmed cell death, 2(1), 101-5. https://doi.org/10.1023/a:1026448027870
Yuan, C C, et al. "Cytokeratin-19 associated with apoptosis and chemosensitivity in human cervical cancer cells." Apoptosis : an international journal on programmed cell death vol. 2,1 (1997): 101-5. doi: https://doi.org/10.1023/a:1026448027870
Yuan CC, Huang HC, Tsai LC, Ng HT, Huang TS. Cytokeratin-19 associated with apoptosis and chemosensitivity in human cervical cancer cells. Apoptosis. 1997;2(1):101-5. doi: 10.1023/a:1026448027870. PMID: 14646570.
Copy Download .nbib Format: NLM
Share it on

Apoptosis. 1997;2(1):101-5. doi: 10.1023/a:1026448027870.

Cytokeratin-19 associated with apoptosis and chemosensitivity in human cervical cancer cells.

Apoptosis : an international journal on programmed cell death

C C Yuan, H C Huang, L C Tsai, H T Ng, T S Huang

Affiliations

  1. School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.

PMID: 14646570 DOI: 10.1023/a:1026448027870

Abstract

Cytokeratins are one group of intermediate filament proteins responsible for the integrity of cell structure, and have been recently reported to play a role in conferring a drug resistance phenotype. MAb Cx-99 is a monoclonal antibody exhibiting the specificity toward its corresponding antigen which was recently identified as the cytokeratin-19 protein. In the present study, we found that the level of cytokeratin-19 in cervical cancer cells could be decreased by incubation of cancer cells with MAb Cx-99. The reduction of cytokeratin-19 level had a killing effect on cervical carcinoma SIHA and HeLa S3 cell lines. The DNA ladder pattern, convoluted nuclei and blebbing morphology were observed with these cells after exposure to MAb Cx-99 for 72 h, suggesting that the cytotoxic mechanism of reduced cytokeratin-19 was mediated by induction of apoptosis. Moreover, the MAb Cx-99 treatment could increase the cytotoxicities of cancer chemotherapeutic agents such as cisplatin and vinblastine to both cervical carcinoma cell lines. The LD80 values were at least 15-fold reduced when cancer cells were treated with cisplatin or vinblastine in the presence of MAb Cx-99. These results suggest that the functional role of cytokeratin-19 was associated with the apoptosis prevention and drug resistance of cervical cancer cells.

Publication Types