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Xu H, Wang D, Zhang W, et al. Determination of isatin and monoamine neurotransmitters in rat brain with liquid chromatography using palladium hexacyanoferrate modified electrode. Anal Chim Acta. 2006;577(2):207-13doi: 10.1016/j.aca.2006.06.042.
Xu, H., Wang, D., Zhang, W., Zhu, W., Yamamoto, K., & Jin, L. (2006). Determination of isatin and monoamine neurotransmitters in rat brain with liquid chromatography using palladium hexacyanoferrate modified electrode. Analytica chimica acta, 577(2), 207-13. https://doi.org/10.1016/j.aca.2006.06.042
Xu, Haihong, et al. "Determination of isatin and monoamine neurotransmitters in rat brain with liquid chromatography using palladium hexacyanoferrate modified electrode." Analytica chimica acta vol. 577,2 (2006): 207-13. doi: https://doi.org/10.1016/j.aca.2006.06.042
Xu H, Wang D, Zhang W, Zhu W, Yamamoto K, Jin L. Determination of isatin and monoamine neurotransmitters in rat brain with liquid chromatography using palladium hexacyanoferrate modified electrode. Anal Chim Acta. 2006 Sep 08;577(2):207-13. doi: 10.1016/j.aca.2006.06.042. Epub 2006 Jun 27. PMID: 17723673.
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Anal Chim Acta. 2006 Sep 08;577(2):207-13. doi: 10.1016/j.aca.2006.06.042. Epub 2006 Jun 27.

Determination of isatin and monoamine neurotransmitters in rat brain with liquid chromatography using palladium hexacyanoferrate modified electrode.

Analytica chimica acta

Haihong Xu, Dan Wang, Wen Zhang, Wei Zhu, Katsunobu Yamamoto, Litong Jin

Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai 200062, PR China.

PMID: 17723673 DOI: 10.1016/j.aca.2006.06.042

Abstract

The fabrication and application of a novel electrochemical detector (ED) with palladium hexacyanoferrate (PdHCF) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behaviors of isatin, monoamine neurotransmitters and their metabolites at this CME were investigated by cyclic voltammetry. It was found that the CME exhibited efficiently electrocatalytic of isatin and showed high sensitivity and stability for determination of monoamine neurotransmitters. The linear ranges were over three orders of magnitude and the detection limits were 2.5 x 10(-8) mol L(-1) for isatin, 2.5 x 10(-10) mol L(-1) for norepinephrine (NE), 2.5 x 10(-10) mol L(-1) for 5-hydroxyindoleacetic acid (5-HIAA), 5.0 x 10(-10) mol L(-1) for dopamine (DA), 1.0 x 10(-9)mol L(-1) for 3,4-dihydroxyphenylacetic acid (DOPAC), 1.2 x 10(-10) mol L(-1) for 5-hydroxytryptamine (5-HT) and 2.5 x 10(-9)mol L(-1) for homovanillic acid (HVA). Combined with microdialysis, the method was successfully applied to study the effect of isatin on the levels of monoamine neurotransmitters in experimental Parkinsonian rats. The results showed that isatin could significantly increase striatal monoamine neurotransmitters release to the basal level.

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