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Chen HK, Chang CK, Wu CC, et al. Synchronized dual-polarity electrospray ionization mass spectrometry. J Am Soc Mass Spectrom. 2009;20(12):2254-7doi: 10.1016/j.jasms.2009.08.014.
Chen, H. K., Chang, C. K., Wu, C. C., Huang, M. C., & Wang, Y. S. (2009). Synchronized dual-polarity electrospray ionization mass spectrometry. Journal of the American Society for Mass Spectrometry, 20(12), 2254-7. https://doi.org/10.1016/j.jasms.2009.08.014
Chen, Han-Kwang, et al. "Synchronized dual-polarity electrospray ionization mass spectrometry." Journal of the American Society for Mass Spectrometry vol. 20,12 (2009): 2254-7. doi: https://doi.org/10.1016/j.jasms.2009.08.014
Chen HK, Chang CK, Wu CC, Huang MC, Wang YS. Synchronized dual-polarity electrospray ionization mass spectrometry. J Am Soc Mass Spectrom. 2009 Dec;20(12):2254-7. doi: 10.1016/j.jasms.2009.08.014. Epub 2009 Aug 28. PMID: 19775909.
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J Am Soc Mass Spectrom. 2009 Dec;20(12):2254-7. doi: 10.1016/j.jasms.2009.08.014. Epub 2009 Aug 28.

Synchronized dual-polarity electrospray ionization mass spectrometry.

Journal of the American Society for Mass Spectrometry

Han-Kwang Chen, Chia-Kai Chang, Chih-Che Wu, Ming-Chia Huang, Yi-Sheng Wang

Affiliations

  1. Department of Chemistry, Chi Nan University, Nantou, Taiwan, Republic of China.

PMID: 19775909 DOI: 10.1016/j.jasms.2009.08.014

Abstract

This work describes the synchronized dual-polarity (DP) electrospray ionization (ESI) method and demonstrates the first DP ESI mass spectra obtained using two mass spectrometers. Stable double Taylor cones were produced by applying two counter electric voltages with opposite polarities to one electrosprayer. The development of double Taylor cones required higher extraction voltages than conventional ESI, but DP ESI worked effectively at liquid flow rate range three times wider than conventional ESI. Using pure methanol, the emission currents of the two cones were neutralized and no current was drawn from the sprayer. Synchronized DP mass spectra were obtained using electrospray calibrants dissolved in methanol solution of low water content. For bovine insulin with conventional electrospray solution, the gas-assisted electrospray delivered satisfactory sensitivity and stability for routine mass analyses.

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