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Di Bella JM, Bao Y, Gloor GB, et al. High throughput sequencing methods and analysis for microbiome research. J Microbiol Methods. 2013;95(3):401-14doi: 10.1016/j.mimet.2013.08.011.
Di Bella, J. M., Bao, Y., Gloor, G. B., Burton, J. P., & Reid, G. (2013). High throughput sequencing methods and analysis for microbiome research. Journal of microbiological methods, 95(3), 401-14. https://doi.org/10.1016/j.mimet.2013.08.011
Di Bella, Julia M, et al. "High throughput sequencing methods and analysis for microbiome research." Journal of microbiological methods vol. 95,3 (2013): 401-14. doi: https://doi.org/10.1016/j.mimet.2013.08.011
Di Bella JM, Bao Y, Gloor GB, Burton JP, Reid G. High throughput sequencing methods and analysis for microbiome research. J Microbiol Methods. 2013 Dec;95(3):401-14. doi: 10.1016/j.mimet.2013.08.011. Epub 2013 Sep 09. PMID: 24029734.
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J Microbiol Methods. 2013 Dec;95(3):401-14. doi: 10.1016/j.mimet.2013.08.011. Epub 2013 Sep 09.

High throughput sequencing methods and analysis for microbiome research.

Journal of microbiological methods

Julia M Di Bella, Yige Bao, Gregory B Gloor, Jeremy P Burton, Gregor Reid

Affiliations

  1. Department of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada.

PMID: 24029734 DOI: 10.1016/j.mimet.2013.08.011

Abstract

High-throughput sequencing technology is rapidly improving in quality, speed and cost. It is therefore becoming more widely used to study whole communities of prokaryotes in many niches. This review discusses these techniques, including nucleic acid extraction from different environments, sample preparation and high-throughput sequencing platforms. We also discuss commonly used and recently developed bioinformatic tools applied to microbiomes, including analyzing amplicon sequences, metagenome shotgun sequences and metatranscriptome sequences. This field is relatively new and rapidly evolving, thus we hope that this review will provide a baseline for understanding these methods of microbiome analyses. Additionally, we seek to stimulate others to solve the many problems that still exist with the sensitivity, specificity and interpretation of high throughput microbiome sequence analysis.

© 2013 Elsevier B.V. All rights reserved.

Keywords: Bioinformatics; High-throughput sequencing; Metagenome; Metatranscriptome; Microbiome

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