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Robinson T, Kuhn P, Eyer K, et al. Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore. Biomicrofluidics. 2013;7(4):44105doi: 10.1063/1.4816712.
Robinson, T., Kuhn, P., Eyer, K., & Dittrich, P. S. (2013). Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore. Biomicrofluidics, 7(4), 44105. https://doi.org/10.1063/1.4816712
Robinson, T, et al. "Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore." Biomicrofluidics vol. 7,4 (2013): 44105. doi: https://doi.org/10.1063/1.4816712
Robinson T, Kuhn P, Eyer K, Dittrich PS. Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore. Biomicrofluidics. 2013 Jul 26;7(4):44105. doi: 10.1063/1.4816712. eCollection 2013. PMID: 24404039; PMCID: PMC3739824.
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Biomicrofluidics. 2013 Jul 26;7(4):44105. doi: 10.1063/1.4816712. eCollection 2013.

Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore.

Biomicrofluidics

T Robinson, P Kuhn, K Eyer, P S Dittrich

Affiliations

  1. Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich 8093, Switzerland.

PMID: 24404039 PMCID: PMC3739824 DOI: 10.1063/1.4816712

Abstract

We present a microfluidic platform able to trap single GUVs in parallel. GUVs are used as model membranes across many fields of biophysics including lipid rafts, membrane fusion, and nanotubes. While their creation is relatively facile, handling and addressing single vesicles remains challenging. The PDMS microchip used herein contains 60 chambers, each with posts able to passively capture single GUVs without compromising their integrity. The design allows for circular valves to be lowered from the channel ceiling to isolate the vesicles from rest of the channel network. GUVs containing calcein were trapped and by rapidly opening the valves, the membrane pore protein α-hemolysin (αHL) was introduced to the membrane. Confocal microscopy revealed the kinetics of the small molecule efflux for different protein concentrations. This microfluidic approach greatly improves the number of experiments possible and can be applied to a wide range of biophysical applications.

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