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Zhao Y, Gao JL, Ji JW, et al. Cytotoxicity enhancement in MDA-MB-231 cells by the combination treatment of tetrahydropalmatine and berberine derived from Corydalis yanhusuo W. T. Wang. J Intercult Ethnopharmacol. 2014;3(2):68-72doi: 10.5455/jice.20140123040224.
Zhao, Y., Gao, J. L., Ji, J. W., Gao, M., Yin, Q. S., Qiu, Q. L., Wang, C., Chen, S. Z., Xu, J., Liang, R. S., Cai, Y. Z., & Wang, X. F. (2014). Cytotoxicity enhancement in MDA-MB-231 cells by the combination treatment of tetrahydropalmatine and berberine derived from Corydalis yanhusuo W. T. Wang. Journal of intercultural ethnopharmacology, 3(2), 68-72. https://doi.org/10.5455/jice.20140123040224
Zhao, Yan, et al. "Cytotoxicity enhancement in MDA-MB-231 cells by the combination treatment of tetrahydropalmatine and berberine derived from Corydalis yanhusuo W. T. Wang." Journal of intercultural ethnopharmacology vol. 3,2 (2014): 68-72. doi: https://doi.org/10.5455/jice.20140123040224
Zhao Y, Gao JL, Ji JW, Gao M, Yin QS, Qiu QL, Wang C, Chen SZ, Xu J, Liang RS, Cai YZ, Wang XF. Cytotoxicity enhancement in MDA-MB-231 cells by the combination treatment of tetrahydropalmatine and berberine derived from Corydalis yanhusuo W. T. Wang. J Intercult Ethnopharmacol. 2014 Apr-Jun;3(2):68-72. doi: 10.5455/jice.20140123040224. Epub 2014 May 27. PMID: 26401350; PMCID: PMC4576799.
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J Intercult Ethnopharmacol. 2014 Apr-Jun;3(2):68-72. doi: 10.5455/jice.20140123040224. Epub 2014 May 27.

Cytotoxicity enhancement in MDA-MB-231 cells by the combination treatment of tetrahydropalmatine and berberine derived from Corydalis yanhusuo W. T. Wang.

Journal of intercultural ethnopharmacology

Yan Zhao, Jian-Li Gao, Jian-Wei Ji, Min Gao, Qiao-Shan Yin, Qiao-Li Qiu, Chuan Wang, Shu-Zhan Chen, Juan Xu, Ren-Shang Liang, Yan-Zi Cai, Xia-Fei Wang

Affiliations

  1. Zhejiang Chinese Medical University, Binjiang, Hangzhou, Zhejiang, China.
  2. Wenzhou Medical University, Wenzhou, Zhejiang, China.

PMID: 26401350 PMCID: PMC4576799 DOI: 10.5455/jice.20140123040224

Abstract

AIM: Our previous works have demonstrated that Chinese herb medicine yanhusuo (Corydalis yanhusuo W. T. Wang) has strong anti-cancer proliferation effect in MDA-MB-231 cells. The goal of this study was to find out the synergic cytotoxicity effect of three natural compounds, tetrahydropalmatine (THP), berberine (Ber), and dehydrocorydaline (DHC), isolated from C. yanhusuo W. T. Wang.

MATERIALS AND METHODS: The IC50 of THP Ber and DHC in single use, as well as in combination use at fixed ratios and doses was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Isobologram, combination index and modified coefficient of drug interaction (CDI) methods were used for evaluation the combination effects of THF! Ber, and DHC in different ratio and concentration.

RESULTS: The results indicated that the combination of THP and Ber shown the strongest anti-cancer cell proliferation effect at the ratio of 2:3 (Ber: THF the average CDI value was 0.5795). DHC and THP have additive cytotoxicity in MDA-MB-231 cells. However, there wasn't any synergistic effect between Ber and DHC, and it even exhibited antagonistic effect when the percentage of DHC was >50%.

CONCLUSION: Our findings suggested that the combination of THP and Ber might be beneficial for anti-proliferation of MDA-MB-231 breast cancer cells through a significant synergy effect.

Keywords: Coefficient of drug interaction; Corydalis Yanhusuo W. T. Wang; combination effect; natural products

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