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Fu XY, Zhang XF, Xia W, et al. Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells. Int J Ophthalmol. 2015;8(5):904-9doi: 10.3980/j.issn.2222-3959.2015.05.09.
Fu, X. Y., Zhang, X. F., Xia, W., Zhong, L., Wang, Y. M., Sun, Z. T., & Xia, J. (2015). Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells. International journal of ophthalmology, 8(5), 904-9. https://doi.org/10.3980/j.issn.2222-3959.2015.05.09
Fu, Xin-Yi, et al. "Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells." International journal of ophthalmology vol. 8,5 (2015): 904-9. doi: https://doi.org/10.3980/j.issn.2222-3959.2015.05.09
Fu XY, Zhang XF, Xia W, Zhong L, Wang YM, Sun ZT, Xia J. Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells. Int J Ophthalmol. 2015 Oct 18;8(5):904-9. doi: 10.3980/j.issn.2222-3959.2015.05.09. eCollection 2015. PMID: 26558199; PMCID: PMC4631012.
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Int J Ophthalmol. 2015 Oct 18;8(5):904-9. doi: 10.3980/j.issn.2222-3959.2015.05.09. eCollection 2015.

Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells.

International journal of ophthalmology

Xin-Yi Fu, Xiao-Feng Zhang, Wei Xia, Lei Zhong, Ying-Ming Wang, Zheng-Tai Sun, Jing Xia

Affiliations

  1. Department of Ophthalmology, the First Affiliated Hospital of Soochow University, Suzhou 215000, Jiangsu Province, China.

PMID: 26558199 PMCID: PMC4631012 DOI: 10.3980/j.issn.2222-3959.2015.05.09

Abstract

AIM: To expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) expression.

METHODS: Three groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were analyzed.

RESULTS: The bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).

CONCLUSION: The expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.

Keywords: Müller cells; basic fibroblast growth factor; monochromatic light; myopia; transforming growth factor-β1

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