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Kumari S, Naik P, Vishma BL, et al. Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity. Cytotechnology. 2015;68(5):1789-800doi: 10.1007/s10616-015-9931-4.
Kumari, S., Naik, P., Vishma, B. L., Salian, S. R., Devkar, R. A., Khan, S., Mutalik, S., Kalthur, G., & Adiga, S. K. (2016). Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity. Cytotechnology, 68(5), 1789-800. https://doi.org/10.1007/s10616-015-9931-4
Kumari, Sandhya, et al. "Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity." Cytotechnology vol. 68,5 (2016): 1789-800. doi: https://doi.org/10.1007/s10616-015-9931-4
Kumari S, Naik P, Vishma BL, Salian SR, Devkar RA, Khan S, Mutalik S, Kalthur G, Adiga SK. Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity. Cytotechnology. 2016 Oct;68(5):1789-800. doi: 10.1007/s10616-015-9931-4. Epub 2015 Nov 21. PMID: 26590833; PMCID: PMC5023552.
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Cytotechnology. 2016 Oct;68(5):1789-800. doi: 10.1007/s10616-015-9931-4. Epub 2015 Nov 21.

Mitigating effect of Indian propolis against mitomycin C induced bone marrow toxicity.

Cytotechnology

Sandhya Kumari, Prashantha Naik, B L Vishma, Sujith Raj Salian, Raviraj Anand Devkar, Saleemulla Khan, Srinivas Mutalik, Guruprasad Kalthur, Satish Kumar Adiga

Affiliations

  1. Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka, 576 104, India.
  2. Department of PG Studies and Research in Biosciences, Mangalore University, Managalagangothri, Karnataka, 574 199, India.
  3. Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, 576 104, India.
  4. Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, Karnataka, 576 104, India. [email protected].

PMID: 26590833 PMCID: PMC5023552 DOI: 10.1007/s10616-015-9931-4

Abstract

A major drawback with cancer chemotherapy is its severe toxic effects on non-target tissues. Assessment of natural products for their protective effect against anticancer drugs-induced toxicity is gaining importance in cancer biology. The present study was aimed at assessing the protective effect of hydroethanolic extract of Indian propolis (HEIP) against mitomycin C (MMC)-induced genotoxicity and cytotoxicity. Swiss albino mice were injected with various doses of HEIP (100, 200, 300, 400, 600 and 800 mg/kg b. wt., i.p) 1 h prior to MMC (8 mg/kg, i.p.) injection. The geno- and cyto-toxicities were evaluated in mice by performing bone marrow micronucleus and TUNEL assays. In vitro antioxidant and lipid peroxidation inhibitory assays were carried out to understand the mechanism of the protective effects. The significant increase in the frequency of micronculeated cells (12.51 ± 0.48), apoptotic cells (23.43 ± 1.86) and reduction in P/N ratio (0.69 ± 0.04) compared with control indicated the potential geno- and cytotoxic effects of MMC in bone marrow. Pretreatment with HEIP resulted in the significant recovery of the toxic effects induced by MMC. HEIP at 400 mg/kg b. wt. was found to be the optimum dose imparting the maximum protective effects. The in vitro antioxidant and lipid peroxidation inhibitory assays suggest that the extract possesses substantial free radical scavenging activities. In conclusion, HEIP possesses substantial geno- and cyto-protective properties against MMC, which could be mediated through efficient free radical scavenging and inhibitory effect on lipid peroxidation.

Keywords: Antioxidant activity; Apoptosis; Genotoxicity; Micronucleus

Conflict of interest statement

Authors declare that there are no conflicts of interest.

References

  1. Mutat Res. 1975 Feb;31(1):9-15 - PubMed
  2. Food Chem Toxicol. 2012 Jun;50(6):2142-8 - PubMed
  3. Pharmazie. 2013 Aug;68(8):695-701 - PubMed
  4. Indian J Exp Biol. 2002 Sep;40(9):1020-5 - PubMed
  5. J Pharm Pharmacol. 2011 Nov;63(11):1378-86 - PubMed
  6. Mutat Res. 2002 Aug 26;519(1-2):37-48 - PubMed
  7. Exp Toxicol Pathol. 2010 Sep;62(5):503-8 - PubMed
  8. J Agric Food Chem. 2013 May 29;61(21):5080-8 - PubMed
  9. Eur J Clin Pharmacol. 2002 Feb;57(12):835-45 - PubMed
  10. Cancer Res. 1985 Aug;45(8):3510-6 - PubMed
  11. Mutagenesis. 1989 Nov;4(6):420-4 - PubMed
  12. Cancer Lett. 1999 Dec 1;147(1-2):221-7 - PubMed
  13. Food Chem Toxicol. 2010 Oct;48(10 ):2741-6 - PubMed
  14. Biochimie. 2002 Dec;84(12 ):1199-205 - PubMed
  15. Indian J Exp Biol. 2014 Feb;52(2):133-8 - PubMed
  16. Molecules. 2013 Dec 20;19(1):78-101 - PubMed
  17. Food Chem Toxicol. 2014 May;67:176-86 - PubMed
  18. Chem Biol Interact. 1989;71(1):63-78 - PubMed
  19. Food Chem Toxicol. 2013 Dec;62:329-42 - PubMed
  20. PLoS One. 2014 Mar 10;9(3):e91588 - PubMed
  21. Anal Biochem. 1999 May 1;269(2):337-41 - PubMed
  22. J Glaucoma. 2014 Feb;23(2):88-94 - PubMed
  23. Asian Pac J Trop Biomed. 2012 Aug;2(8):651-4 - PubMed
  24. J Pharm Biomed Anal. 2013 Jul-Aug;81-82:126-32 - PubMed
  25. Mutat Res. 1987 Oct;189(2):103-12 - PubMed
  26. Biomed Environ Sci. 2004 Dec;17(4):469-75 - PubMed
  27. Biochem Soc Symp. 2004;(71):157-76 - PubMed
  28. Pediatr Hematol Oncol. 2012 Apr;29(3):285-92 - PubMed
  29. J Agric Food Chem. 2000 Apr;48(4):1129-34 - PubMed
  30. Cancer Treat Rev. 1976 Sep;3(3):121-39 - PubMed
  31. Anal Biochem. 1996 Jul 15;239(1):70-6 - PubMed
  32. Oxid Med Cell Longev. 2010 Jul-Aug;3(4):228-37 - PubMed
  33. Phytother Res. 2009 Aug;23(8):1159-68 - PubMed
  34. Toxicol Ind Health. 2013 Sep;29(8):677-85 - PubMed
  35. Am J Chin Med. 2002;30(2-3):245-54 - PubMed
  36. An Acad Bras Cienc. 2013 Sep;85(3):955-64 - PubMed
  37. Indian J Exp Biol. 2008 Feb;46(2):112-9 - PubMed
  38. Int J Exp Pathol. 2005 Dec;86(6):415-30 - PubMed
  39. Mutagenesis. 1991 Jul;6(4):297-302 - PubMed
  40. J Biomed Biotechnol. 2009;2009:791432 - PubMed

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