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Ke J, Ma H, Gu X, et al. Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors. Sci Adv. 2015;1(6):e1500107doi: 10.1126/sciadv.1500107.
Ke, J., Ma, H., Gu, X., Thelen, A., Brunzelle, J. S., Li, J., Xu, H. E., & Melcher, K. (2015). Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors. Science advances, 1(6), e1500107. https://doi.org/10.1126/sciadv.1500107
Ke, Jiyuan, et al. "Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors." Science advances vol. 1,6 (2015): e1500107. doi: https://doi.org/10.1126/sciadv.1500107
Ke J, Ma H, Gu X, Thelen A, Brunzelle JS, Li J, Xu HE, Melcher K. Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors. Sci Adv. 2015 Jul 24;1(6):e1500107. doi: 10.1126/sciadv.1500107. eCollection 2015 Jul. PMID: 26601214; PMCID: PMC4646777.
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Sci Adv. 2015 Jul 24;1(6):e1500107. doi: 10.1126/sciadv.1500107. eCollection 2015 Jul.

Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors.

Science advances

Jiyuan Ke, Honglei Ma, Xin Gu, Adam Thelen, Joseph S Brunzelle, Jiayang Li, H Eric Xu, Karsten Melcher

Affiliations

  1. Key Laboratory of Receptor Research, VARI-SIMM Center, Center for Structure and Function of Drug Targets, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China. ; Laboratory of Structural Sciences and Laboratory of Structural Biology and Biochemistry, Van Andel Research Institute, 333 Bostwick Avenue Northeast, Grand Rapids, MI 49503, USA.
  2. Laboratory of Structural Sciences and Laboratory of Structural Biology and Biochemistry, Van Andel Research Institute, 333 Bostwick Avenue Northeast, Grand Rapids, MI 49503, USA.
  3. Department of Molecular Pharmacology and Biological Chemistry, Life Sciences Collaborative Access Team, Synchrotron Research Center, Northwestern University, Argonne, IL 60439, USA.
  4. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.

PMID: 26601214 PMCID: PMC4646777 DOI: 10.1126/sciadv.1500107

Abstract

TOPLESS (TPL) and TOPLESS-related (TPR) proteins comprise a conserved family of plant transcriptional corepressors that are related to Tup1, Groucho, and TLE (transducin-like enhancer of split) corepressors in yeast, insects, and mammals. In plants, TPL/TPR corepressors regulate development, stress responses, and hormone signaling through interaction with small ethylene response factor-associated amphiphilic repression (EAR) motifs found in diverse transcriptional repressors. How EAR motifs can interact with TPL/TPR proteins is unknown. We confirm the amino-terminal domain of the TPL family of corepressors, which we term TOPLESS domain (TPD), as the EAR motif-binding domain. To understand the structural basis of this interaction, we determined the crystal structures of the TPD of rice (Os) TPR2 in apo (apo protein) state and in complexes with the EAR motifs from Arabidopsis NINJA (novel interactor of JAZ), IAA1 (auxin-responsive protein 1), and IAA10, key transcriptional repressors involved in jasmonate and auxin signaling. The OsTPR2 TPD adopts a new fold of nine helices, followed by a zinc finger, which are arranged into a disc-like tetramer. The EAR motifs in the three different complexes adopt a similar extended conformation with the hydrophobic residues fitting into the same surface groove of each OsTPR2 monomer. Sequence alignments and structure-based mutagenesis indicate that this mode of corepressor binding is highly conserved in a large set of transcriptional repressors, thus providing a general mechanism for gene repression mediated by the TPL family of corepressors.

Keywords: EAR; Groucho; IAA; NINJA; TLE; TPD; TPR; TUP1; Topless; auxin; jasmonate

References

  1. Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11786-91 - PubMed
  2. Genes Dev. 2001 Feb 1;15(3):261-6 - PubMed
  3. Genome Biol. 2008 Jan 31;9(1):205 - PubMed
  4. Science. 2011 Jul 29;333(6042):601-7 - PubMed
  5. Biochem Biophys Res Commun. 2004 Aug 13;321(1):172-8 - PubMed
  6. Genes Dev. 1994 Dec 1;8(23):2857-67 - PubMed
  7. Cell. 1994 Dec 2;79(5):805-15 - PubMed
  8. Curr Opin Plant Biol. 2013 Oct;16(5):561-8 - PubMed
  9. Curr Opin Plant Biol. 2009 Oct;12(5):628-36 - PubMed
  10. Plant Cell. 2014 Jul;26(7):2920-38 - PubMed
  11. EMBO J. 2014 Apr 1;33(7):719-31 - PubMed
  12. Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67 - PubMed
  13. Plant J. 2012 Apr;70(2):327-39 - PubMed
  14. Plant Cell. 2004 Feb;16(2):533-43 - PubMed
  15. Science. 2008 Mar 7;319(5868):1384-6 - PubMed
  16. J Biol Chem. 2008 Aug 15;283(33):22637-48 - PubMed
  17. Biochem Cell Biol. 2006 Aug;84(4):437-43 - PubMed
  18. Nature. 1994 Jun 30;369(6483):758-61 - PubMed
  19. Nat Commun. 2014 Apr 07;5:3617 - PubMed
  20. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13839-44 - PubMed
  21. Plant J. 2009 Jul;59(1):77-87 - PubMed
  22. Nature. 2009 Dec 3;462(7273):602-8 - PubMed
  23. Nat Commun. 2014 Jun 18;5:4140 - PubMed
  24. Trends Cell Biol. 2007 Jul;17(7):353-61 - PubMed
  25. Development. 2012 Nov;139(22):4180-90 - PubMed
  26. Nature. 2007 Aug 9;448(7154):666-71 - PubMed
  27. Nature. 2013 Dec 19;504(7480):406-10 - PubMed
  28. Development. 1999 Sep;126(17):3747-55 - PubMed
  29. Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55 - PubMed
  30. Nature. 1998 Oct 8;395(6702):608-12 - PubMed
  31. Plant Physiol. 2010 Mar;152(3):1109-34 - PubMed
  32. Mol Cell Biol. 2004 May;24(10):4341-50 - PubMed
  33. Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15771-6 - PubMed
  34. Science. 2006 Jun 9;312(5779):1520-3 - PubMed
  35. Epigenetics. 2011 Feb;6(2):141-6 - PubMed
  36. Plant Physiol. 2012 Jan;158(1):423-38 - PubMed
  37. Genes Dev. 2013 Nov 1;27(21):2305-19 - PubMed
  38. Cell. 2001 Mar 23;104(6):861-73 - PubMed
  39. Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13960-5 - PubMed
  40. Hum Mol Genet. 2001 Nov 15;10 (24):2813-20 - PubMed
  41. Nucleic Acids Res. 1998 Mar 15;26(6):1414-20 - PubMed
  42. EMBO J. 2006 Jun 21;25(12):2930-9 - PubMed
  43. Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):582-601 - PubMed
  44. Plant Cell. 2009 Jan;21(1):131-45 - PubMed
  45. Nature. 2010 Apr 1;464(7289):788-91 - PubMed
  46. Trends Plant Sci. 2008 Mar;13(3):137-44 - PubMed
  47. Mol Cell. 2007 Oct 26;28(2):291-303 - PubMed
  48. Nature. 2013 Dec 19;504(7480):401-5 - PubMed
  49. Nature. 2007 Aug 9;448(7154):661-5 - PubMed
  50. Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):760-3 - PubMed
  51. J Biol Chem. 2012 Aug 3;287(32):26528-38 - PubMed
  52. Plant Cell. 2012 Feb;24(2):536-50 - PubMed
  53. Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32 - PubMed
  54. Genes Dev. 1997 Nov 15;11(22):3072-82 - PubMed
  55. Nucleic Acids Res. 2001 Apr 1;29(7):1410-9 - PubMed
  56. Mol Cell Biol. 2007 Aug;27(15):5306-15 - PubMed
  57. Proc Natl Acad Sci U S A. 2014 Dec 30;111(52):18613-8 - PubMed
  58. Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32 - PubMed
  59. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5427-32 - PubMed
  60. Curr Opin Plant Biol. 2014 Oct;21:51-8 - PubMed

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