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Han D, Wan C, Liu F, et al. Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3K/Akt/mTOR Signaling Pathway. Evid Based Complement Alternat Med. 2016;2016:9593716doi: 10.1155/2016/9593716.
Han, D., Wan, C., Liu, F., Xu, X., Jiang, L., & Xu, J. (2016). Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3K/Akt/mTOR Signaling Pathway. Evidence-based complementary and alternative medicine : eCAM, 20169593716. https://doi.org/10.1155/2016/9593716
Han, Dandan, et al. "Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3K/Akt/mTOR Signaling Pathway." Evidence-based complementary and alternative medicine : eCAM vol. 2016 (2016): 9593716. doi: https://doi.org/10.1155/2016/9593716
Han D, Wan C, Liu F, Xu X, Jiang L, Xu J. Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3K/Akt/mTOR Signaling Pathway. Evid Based Complement Alternat Med. 2016;2016:9593716. doi: 10.1155/2016/9593716. Epub 2016 May 16. PMID: 27293469; PMCID: PMC4884826.
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Evid Based Complement Alternat Med. 2016;2016:9593716. doi: 10.1155/2016/9593716. Epub 2016 May 16.

Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3K/Akt/mTOR Signaling Pathway.

Evidence-based complementary and alternative medicine : eCAM

Dandan Han, Changrong Wan, Fenghua Liu, Xiaolong Xu, Linshu Jiang, Jianqin Xu

Affiliations

  1. CAU-BUA TCVM Teaching and Researching Team, College of Veterinary Medicine, China Agricultural University (CAU), Beijing 100193, China.
  2. Beijing Key Laboratory of Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture (BUA), Beijing 102206, China.
  3. Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China.

PMID: 27293469 PMCID: PMC4884826 DOI: 10.1155/2016/9593716

Abstract

Jujuboside A is a kind of the saponins isolated from the seeds of Ziziphus jujuba, which possesses multiple biological effects, such as antianxiety, antioxidant, and anti-inflammatory effects; however, its mediatory effect on isoproterenol-stimulated cardiomyocytes has not been investigated yet. In this study, we tried to detect the protective effect and potential mechanism of JUA on ISO-induced cardiomyocytes injury. H9C2 cells were treated with ISO to induce cell damage. Cells were pretreated with JUA to investigate the effects on the cell viability, morphological changes, light chain 3 conversion, and the activation of PI3K/Akt/mTOR signaling pathway. Results showed that ISO significantly inhibited the cell viability in a time- and dose-dependent manner. JUA pretreatment could reverse the reduction of cell viability and better the injury of H9C2 cells induced by ISO. Western blot analysis showed that JUA could accelerate the phosphorylation of PI3K, Akt, and mTOR. Results also indicated that JUA could significantly decrease the ratio of microtubule-associated protein LC3-II/I in H9C2 cells. Taken together, our research showed that JUA could notably reduce the damage cause by ISO via promoting the phosphorylation of PI3K, Akt, and mTOR and inhibiting LC3 conversion, which may be a potential choice for the treatment of heart diseases.

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