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Lee JY, Park S, Kim KS, et al. Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells. Dev Reprod. 2016;20(2):171-7doi: 10.12717/DR.2016.20.2.171.
Lee, J. Y., Park, S., Kim, K. S., Ko, J. J., Lee, S., Kim, K. P., & Park, K. S. (2016). Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells. Development & reproduction, 20(2), 171-7. https://doi.org/10.12717/DR.2016.20.2.171
Lee, Jae-Young, et al. "Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells." Development & reproduction vol. 20,2 (2016): 171-7. doi: https://doi.org/10.12717/DR.2016.20.2.171
Lee JY, Park S, Kim KS, Ko JJ, Lee S, Kim KP, Park KS. Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells. Dev Reprod. 2016 Jun;20(2):171-7. doi: 10.12717/DR.2016.20.2.171. PMID: 27660833; PMCID: PMC5027223.
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Dev Reprod. 2016 Jun;20(2):171-7. doi: 10.12717/DR.2016.20.2.171.

Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells.

Development & reproduction

Jae-Young Lee, Sunghyun Park, Kwang-Soo Kim, Jeong-Jae Ko, Soohong Lee, Keun Pil Kim, Kyung-Soon Park

Affiliations

  1. Dept. of Biomedical Science, College of Life Science, CHA University, Seoul 06135, Korea.
  2. Dept. of Life Science, Chung-Ang University, Seoul 06975, Korea.

PMID: 27660833 PMCID: PMC5027223 DOI: 10.12717/DR.2016.20.2.171

Abstract

Sprouty (Spry) genes encode inhibitors of the receptor tyrosine kinase signaling cascade, which plays important roles in stem cells. However, the role of Spry4 in the stemness of embryonic stem cells has not been fully elucidated. Here, we used mouse embryonic stem cells (mESCs) as a model system to investigate the role of Spry4 in the stem cells. Suppression of Spry4 expression results in the decreases of cell proliferation, EB formation and stemness marker expression, suggesting that Spry4 activity is associated with stemness of mESCs. Teratoma assay showed that the cartilage maturation was facilitated in Spry4 knocked down mESCs. Our results suggest that Spry4 is an important regulator of the stemness and differentiation of mESCs.

Keywords: Differentiation; Embryonic stem cell; Sprouty4

References

  1. PLoS One. 2009;4(5):e5467 - PubMed
  2. Development. 1999 Jun;126(11):2515-25 - PubMed
  3. Mol Cell Biol. 1998 Feb;18(2):790-8 - PubMed
  4. Dev Cell. 2007 Jun;12(6):931-41 - PubMed
  5. Eur J Biochem. 2002 May;269(10):2546-56 - PubMed
  6. Cell. 1998 Jan 23;92(2):253-63 - PubMed
  7. Circ Res. 2008 Oct 10;103(8):784-95 - PubMed
  8. Tissue Eng Part A. 2012 Oct;18(19-20):1949-56 - PubMed
  9. Cancer Sci. 2009 Sep;100(9):1648-54 - PubMed
  10. J Biol Chem. 2001 Sep 28;276(39):36804-8 - PubMed
  11. Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6041-6 - PubMed
  12. Biol Chem. 2010 Jul;391(7):813-21 - PubMed
  13. J Cell Biol. 2001 Mar 5;152(5):1087-98 - PubMed
  14. J Biol Chem. 2001 Feb 23;276(8):5866-75 - PubMed
  15. Mech Dev. 1999 Mar;81(1-2):213-6 - PubMed
  16. J Biol Chem. 2001 Feb 9;276(6):4128-33 - PubMed
  17. Angiogenesis. 2008;11(1):53-62 - PubMed
  18. Cell Death Differ. 2007 Oct;14 (10 ):1802-12 - PubMed
  19. Blood. 2003 Sep 15;102(6):2074-80 - PubMed
  20. Development. 2007 Aug;134(16):2895-902 - PubMed
  21. Development. 2010 Mar;137(6):881-90 - PubMed
  22. Development. 2007 Aug;134(16):2889-94 - PubMed
  23. EMBO J. 2002 Sep 16;21(18):4796-808 - PubMed
  24. Curr Biol. 2003 Feb 18;13(4):297-307 - PubMed
  25. Sci Rep. 2013;3:2277 - PubMed
  26. Osteoarthritis Cartilage. 2012 Oct;20(10):1170-8 - PubMed
  27. Stem Cells Dev. 2012 Mar 1;21(4):554-61 - PubMed
  28. Cancer Sci. 2007 Jun;98(6):815-21 - PubMed
  29. J Biol Chem. 2001 Dec 7;276(49):46460-8 - PubMed
  30. Nature. 2008 Jun 5;453(7196):733-5 - PubMed
  31. Cell Prolif. 2010 Aug;43(4):333-43 - PubMed

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