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Cobice DF, Livingstone DE, Mackay CL, et al. Spatial Localization and Quantitation of Androgens in Mouse Testis by Mass Spectrometry Imaging. Anal Chem. 2016;88(21):10362-10367doi: 10.1021/acs.analchem.6b02242.
Cobice, D. F., Livingstone, D. E., Mackay, C. L., Goodwin, R. J., Smith, L. B., Walker, B. R., & Andrew, R. (2016). Spatial Localization and Quantitation of Androgens in Mouse Testis by Mass Spectrometry Imaging. Analytical chemistry, 88(21), 10362-10367. https://doi.org/10.1021/acs.analchem.6b02242
Cobice, Diego F, et al. "Spatial Localization and Quantitation of Androgens in Mouse Testis by Mass Spectrometry Imaging." Analytical chemistry vol. 88,21 (2016): 10362-10367. doi: https://doi.org/10.1021/acs.analchem.6b02242
Cobice DF, Livingstone DE, Mackay CL, Goodwin RJ, Smith LB, Walker BR, Andrew R. Spatial Localization and Quantitation of Androgens in Mouse Testis by Mass Spectrometry Imaging. Anal Chem. 2016 Nov 01;88(21):10362-10367. doi: 10.1021/acs.analchem.6b02242. Epub 2016 Oct 14. PMID: 27676129; PMCID: PMC5102453.
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Anal Chem. 2016 Nov 01;88(21):10362-10367. doi: 10.1021/acs.analchem.6b02242. Epub 2016 Oct 14.

Spatial Localization and Quantitation of Androgens in Mouse Testis by Mass Spectrometry Imaging.

Analytical chemistry

Diego F Cobice, Dawn E W Livingstone, C Logan Mackay, Richard J A Goodwin, Lee B Smith, Brian R Walker, Ruth Andrew

Affiliations

  1. University/British Heart Foundation Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh , 47 Little France Crescent, Edinburgh, EH16 4TJ, United Kingdom.
  2. Centre for Integrative Physiology, University of Edinburgh , Hugh Robson Building, 15 George Square, Edinburgh EH8 9XD, United Kingdom.
  3. SIRCAMS, School of Chemistry, University of Edinburgh , Joseph Black Building, The King's Buildings, West Mains Road, Edinburgh, EH9 3JJ, United Kingdom.
  4. AstraZeneca R&D , Cambridge, CB4 0WG, United Kingdom.
  5. MRC Centre for Reproductive Health; Queen's Medical Research Institute, University of Edinburgh , 47 Little France Crescent, Edinburgh, EH16 4TJ, United Kingdom.

PMID: 27676129 PMCID: PMC5102453 DOI: 10.1021/acs.analchem.6b02242

Abstract

Androgens are essential for male development and reproductive function. They are transported to their site of action as blood-borne endocrine hormones but can also be produced within tissues to act in intracrine and paracrine fashions. Because of this, circulating concentrations may not accurately reflect the androgenic influence within specific tissue microenvironments. Mass spectrometry imaging permits regional analysis of small molecular species directly from tissue surfaces. However, due to poor ionization and localized ion suppression, steroid hormones are difficult to detect. Here, derivatization with Girard T reagent was used to charge-tag testosterone and 5α-dihydrotestosterone allowing direct detection of these steroids in mouse testes, in both basal and maximally stimulated states, and in rat prostate. Limits of detection were ∼0.1 pg for testosterone. Exemplary detection of endogenous steroids was achieved by matrix-assisted laser desorption ionization and either Fourier transform ion cyclotron resonance detection (at 150 μm spatial resolution) or quadrupole-time-of-flight detection (at 50 μm spatial resolution). Structural confirmation was achieved by collision induced fragmentation following liquid extraction surface analysis and electrospray ionization. This application broadens the scope for derivatization strategies on tissue surfaces to elucidate local endocrine signaling in health and disease.

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