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Rezhdo O, Di Maio S, Le P, et al. Characterization of colloidal structures during intestinal lipolysis using small-angle neutron scattering. J Colloid Interface Sci. 2017;499:189-201doi: 10.1016/j.jcis.2017.03.109.
Rezhdo, O., Di Maio, S., Le, P., Littrell, K. C., Carrier, R. L., & Chen, S. H. (2017). Characterization of colloidal structures during intestinal lipolysis using small-angle neutron scattering. Journal of colloid and interface science, 499189-201. https://doi.org/10.1016/j.jcis.2017.03.109
Rezhdo, Oljora, et al. "Characterization of colloidal structures during intestinal lipolysis using small-angle neutron scattering." Journal of colloid and interface science vol. 499 (2017): 189-201. doi: https://doi.org/10.1016/j.jcis.2017.03.109
Rezhdo O, Di Maio S, Le P, Littrell KC, Carrier RL, Chen SH. Characterization of colloidal structures during intestinal lipolysis using small-angle neutron scattering. J Colloid Interface Sci. 2017 Aug 01;499:189-201. doi: 10.1016/j.jcis.2017.03.109. Epub 2017 Mar 30. PMID: 28384537; PMCID: PMC5627765.
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J Colloid Interface Sci. 2017 Aug 01;499:189-201. doi: 10.1016/j.jcis.2017.03.109. Epub 2017 Mar 30.

Characterization of colloidal structures during intestinal lipolysis using small-angle neutron scattering.

Journal of colloid and interface science

Oljora Rezhdo, Selena Di Maio, Peisi Le, Kenneth C Littrell, Rebecca L Carrier, Sow-Hsin Chen

Affiliations

  1. Department of Chemical Engineering, Northeastern University, 360 Huntington Ave., Boston, MA 02151, USA. Electronic address: [email protected].
  2. Department of Medicine, University of California San Francisco, 405 Parnassus Ave., San Francisco, CA 94143, USA. Electronic address: [email protected].
  3. Department of Nuclear Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139, USA. Electronic address: [email protected].
  4. High Flux Isotope Reactor, Oak Ridge National Laboratory, 1 Bethel Valley Rd., Oak Ridge, TN 37831, USA. Electronic address: [email protected].
  5. Department of Chemical Engineering, Northeastern University, 360 Huntington Ave., Boston, MA 02151, USA. Electronic address: [email protected].
  6. Department of Nuclear Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139, USA. Electronic address: [email protected].

PMID: 28384537 PMCID: PMC5627765 DOI: 10.1016/j.jcis.2017.03.109

Abstract

HYPOTHESIS: Bile micelles are thought to mediate intestinal absorption, in part by providing a phase into which compounds can partition. Solubilizing capacity of bile micelles is enhanced during the digestion of fat rich food. We hypothesized that the intestinal digestion of triglycerides causes an increase in volume of micelles that can be quantitatively monitored over the course of digestion using small-angle neutron scattering (SANS), and that SANS can enable evaluation of the contribution of each of the components present during digestion to the size of micelles.

EXPERIMENTS: SANS was used to characterize the size and shape of micelles present prior to and during the in vitro simulated intestinal digestion of a model food-associated lipid, triolein.

FINDINGS: Pre-lipolysis mixtures of a bile salt and phospholipid simulating bile concentrations in fed conditions were organized in micelles with an average volume of 40 nm

Copyright © 2017 Elsevier Inc. All rights reserved.

Keywords: Bile micelles; Colloidal structures; Digestion; Diglyceride; Fatty acid; Monoglyceride; Simulated intestinal fluids; Small-angle neutron scattering; Triglyceride

References

  1. J Chromatogr A. 2014 Nov 21;1369:105-15 - PubMed
  2. Mol Pharm. 2012 Feb 6;9(2):237-47 - PubMed
  3. Food Funct. 2012 May;3(5):537-46 - PubMed
  4. Int J Pharm. 2013 Aug 30;453(1):44-55 - PubMed
  5. Biochemistry. 1980 Feb 19;19(4):601-15 - PubMed
  6. Adv Drug Deliv Rev. 2008 Mar 17;60(6):617-24 - PubMed
  7. J Pharm Sci. 2003 Mar;92(3):634-48 - PubMed
  8. J Colloid Interface Sci. 2015 Jul 1;449:160-6 - PubMed
  9. Eur J Pharm Biopharm. 2015 Oct;96:117-24 - PubMed
  10. Drug Discov Today. 2010 Nov;15(21-22):958-65 - PubMed
  11. Clin Gastroenterol Hepatol. 2005 Jan;3(1):28-38 - PubMed
  12. Nat Rev Drug Discov. 2007 Mar;6(3):231-48 - PubMed
  13. Eur J Pharm Biopharm. 2014 Apr;86(3):427-37 - PubMed
  14. Eur J Pharm Sci. 2007 Jun;31(2):85-94 - PubMed
  15. J Pharm Pharm Sci. 2005 Jul 08;8(2):147-65 - PubMed
  16. Biochemistry. 1988 Mar 22;27(6):1881-8 - PubMed
  17. Langmuir. 2011 Aug 2;27(15):9528-34 - PubMed
  18. Mol Pharm. 2016 Oct 3;13(10 ):3484-3493 - PubMed
  19. Molecules. 2007 Oct 23;12(10):2292-326 - PubMed
  20. Clin Pharmacokinet. 1999 Sep;37(3):213-55 - PubMed
  21. Mol Pharm. 2014 Aug 4;11(8):2825-34 - PubMed
  22. J Control Release. 2011 Apr 30;151(2):110-22 - PubMed
  23. Front Biosci. 2001 Mar 01;6:D299-319 - PubMed
  24. J Colloid Interface Sci. 2002 Dec 1;256(1):201-7 - PubMed
  25. Chem Phys Lipids. 2001 May;111(1):73-85 - PubMed
  26. J Pharm Pharmacol. 2010 Nov;62(11):1622-36 - PubMed
  27. J Pharm Sci. 2004 Feb;93(2):332-48 - PubMed
  28. J Biol Chem. 1980 Jun 10;255(11):5064-8 - PubMed
  29. Pharm Res. 2013 Dec;30(12):3088-100 - PubMed
  30. Pharm Res. 2006 Apr;23(4):742-51 - PubMed
  31. Langmuir. 2010 Jul 20;26(14 ):11670-9 - PubMed
  32. Pharm Res. 2013 Dec;30(12):3131-44 - PubMed
  33. Biochim Biophys Acta. 2008 Aug;1781(8):367-75 - PubMed
  34. Biochim Biophys Acta. 1966 Dec 7;125(3):563-80 - PubMed
  35. Adv Colloid Interface Sci. 2011 Jun 9;165(1):36-46 - PubMed
  36. Phys Chem Chem Phys. 2011 Feb 28;13(8):3171-8 - PubMed
  37. J Phys Chem B. 2010 Dec 16;114(49):16414-21 - PubMed
  38. Crit Rev Ther Drug Carrier Syst. 2014;31(2):121-85 - PubMed
  39. J Pharm Sci. 2005 Mar;94(3):481-92 - PubMed
  40. J Pharm Biomed Anal. 2001 Jun;25(3-4):651-61 - PubMed
  41. J Pharm Pharmacol. 2002 Jan;54(1):29-41 - PubMed

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