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Ding C, Yang C, Cao Q, et al. Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes. Exp Ther Med. 2017;14(3):2108-2116doi: 10.3892/etm.2017.4789.
Ding, C., Yang, C., Cao, Q., Zhu, X., Zhang, J., Zhang, W., Wang, Y., & Li, L. (2017). Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes. Experimental and therapeutic medicine, 14(3), 2108-2116. https://doi.org/10.3892/etm.2017.4789
Ding, Chandong, et al. "Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes." Experimental and therapeutic medicine vol. 14,3 (2017): 2108-2116. doi: https://doi.org/10.3892/etm.2017.4789
Ding C, Yang C, Cao Q, Zhu X, Zhang J, Zhang W, Wang Y, Li L. Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes. Exp Ther Med. 2017 Sep;14(3):2108-2116. doi: 10.3892/etm.2017.4789. Epub 2017 Jul 12. PMID: 28962131; PMCID: PMC5609193.
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Exp Ther Med. 2017 Sep;14(3):2108-2116. doi: 10.3892/etm.2017.4789. Epub 2017 Jul 12.

Effect of mHCN2 gene modification on chronotropic relevant receptors in BMSCs co-cultured with atrial myocytes.

Experimental and therapeutic medicine

Chandong Ding, Cuicui Yang, Quanxia Cao, Xiaoxia Zhu, Jianming Zhang, Wen Zhang, Yongping Wang, Long Li

Affiliations

  1. Department of Cardiology, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.
  2. Catheter Intervention Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China.

PMID: 28962131 PMCID: PMC5609193 DOI: 10.3892/etm.2017.4789

Abstract

Currently, the mechanism of the chronotropic ability of stem cells modified to express the hyperpolarization-activated cyclic nucleotide-gated (HCN) gene remains to be elucidated. The present study assessed the effects of mouse (m)HCN2 gene modification on the expression of chronotropic relevant receptors, adrenergic receptor β1 (Adrb1) and cholinergic receptor muscarinic M2 (Chrm2), in bone marrow stromal cells (BMSCs) co-cultured with atrial myocytes. BMSCs were divided into the following four groups: i) BMSCs transfected with the mHCN2 gene and co-cultured with atrial myocytes for 48 h (TF + CO); ii) respective transfection (TF); iii) respective co-culture (CO); and iv) the control group without treatment (CTL). Green fluorescent protein (GFP) was observed in the BMSCs 48 h after transfection with pEGFP-C1-mHCN2. The expression of Adrb1 and Chrm2 was significantly increased in the TF and TF + CO groups, particularly the TF + CO group, compared with the CTL group (P<0.05). This suggests that BMSCs modified to express the mHCN2 gene possess autorhythmicity and chronotropic ability, particularly when co-cultured with atrial myocytes. The results of the present study provide novel information regarding the molecular basis of biological pacemakers' chronotropic ability.

Keywords: adrenergic receptor β1; chronotropic ability; gene modification; hyperpolarization-activated cyclic nucleotide-gated; stem cells

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