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Zheng H, Chen M, Li Y, et al. Modulation of Gut Microbiome Composition and Function in Experimental Colitis Treated with Sulfasalazine. Front Microbiol. 2017;8:1703doi: 10.3389/fmicb.2017.01703.
Zheng, H., Chen, M., Li, Y., Wang, Y., Wei, L., Liao, Z., Wang, M., Ma, F., Liao, Q., & Xie, Z. (2017). Modulation of Gut Microbiome Composition and Function in Experimental Colitis Treated with Sulfasalazine. Frontiers in microbiology, 81703. https://doi.org/10.3389/fmicb.2017.01703
Zheng, Haihui, et al. "Modulation of Gut Microbiome Composition and Function in Experimental Colitis Treated with Sulfasalazine." Frontiers in microbiology vol. 8 (2017): 1703. doi: https://doi.org/10.3389/fmicb.2017.01703
Zheng H, Chen M, Li Y, Wang Y, Wei L, Liao Z, Wang M, Ma F, Liao Q, Xie Z. Modulation of Gut Microbiome Composition and Function in Experimental Colitis Treated with Sulfasalazine. Front Microbiol. 2017 Sep 07;8:1703. doi: 10.3389/fmicb.2017.01703. eCollection 2017. PMID: 28936203; PMCID: PMC5594074.
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Front Microbiol. 2017 Sep 07;8:1703. doi: 10.3389/fmicb.2017.01703. eCollection 2017.

Modulation of Gut Microbiome Composition and Function in Experimental Colitis Treated with Sulfasalazine.

Frontiers in microbiology

Haihui Zheng, Mingyi Chen, Yuan Li, Yuanyuan Wang, Lin Wei, Ziqiong Liao, Mengxia Wang, Fangli Ma, Qiongfeng Liao, Zhiyong Xie

Affiliations

  1. School of Pharmaceutical Sciences, Sun Yat-sen UniversityGuangzhou, China.
  2. School of Chinese Materia Medica, Guangzhou University of Chinese MedicineGuangzhou, China.
  3. Infinitus (China) Company Ltd.Guangzhou, China.

PMID: 28936203 PMCID: PMC5594074 DOI: 10.3389/fmicb.2017.01703

Abstract

Inflammatory bowel disease (IBD) results from alterations in intestinal flora and the immune system. Sulfasalazine (SASP) is a sulfa antimicrobial used to treat IBD in clinic for years. However, how SASP affects gut microbes and its potential functions remains unclear. To investigate the relationships of SASP, IBD, and gut microbiome, we used 2,4,6-trinitrobenzenesulfonic acid (TNBS) to induce experimental colitis in rats, and analyzed the microbiota in the fecal samples, which come from the control group (treated with ethanol + saline), the model group (treated with TNBS-ethanol + saline) and the SASP group (treated with TNBS-ethanol + SASP), with 16S gene sequencing and followed up a subset sample using shotgun sequencing. The study found that SASP treatment could not only restore the TNBS-induced gut dysbiosis, which was proved by the increasing amount of SCFAs-producing bacteria and lactic acid-producing bacteria as well as the decreasing amount of Proteobacteria, but also modulate the dysregulated function of the TNBS-induced colitis to resemble that of the control group, including an increased capacity for basic metabolism (carbohydrate metabolism, citrate cycle) and a decrease in the oxidative stress (riboflavin, sulfur, cysteine) as well as bacterial pathogenesis (cell motility and secretion, bacterial motility proteins, flagellar assembly). Moreover, a higher proportion of

Keywords: 16S gene sequencing; SASP; TNBS; colitis; gut microbiome; metagenomics

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