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Rickel JMR, Dixon AJ, Klibanov AL, et al. A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles. Lab Chip. 2018;18(17):2653-2664doi: 10.1039/c8lc00496j.
Rickel, J. M. R., Dixon, A. J., Klibanov, A. L., & Hossack, J. A. (2018). A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles. Lab on a chip, 18(17), 2653-2664. https://doi.org/10.1039/c8lc00496j
Rickel, J M Robert, et al. "A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles." Lab on a chip vol. 18,17 (2018): 2653-2664. doi: https://doi.org/10.1039/c8lc00496j
Rickel JMR, Dixon AJ, Klibanov AL, Hossack JA. A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles. Lab Chip. 2018 Aug 21;18(17):2653-2664. doi: 10.1039/c8lc00496j. PMID: 30070301; PMCID: PMC6566100.
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Lab Chip. 2018 Aug 21;18(17):2653-2664. doi: 10.1039/c8lc00496j.

A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles.

Lab on a chip

J M Robert Rickel, Adam J Dixon, Alexander L Klibanov, John A Hossack

Affiliations

  1. Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA. [email protected].

PMID: 30070301 PMCID: PMC6566100 DOI: 10.1039/c8lc00496j

Abstract

Flow focusing microfluidic devices (FFMDs) have been investigated for the production of monodisperse populations of microbubbles for chemical, biomedical and mechanical engineering applications. High-speed optical microscopy is commonly used to monitor FFMD microbubble production parameters, such as diameter and production rate, but this limits the scalability and portability of the approach. In this work, a novel FFMD design featuring integrated electronics for measuring microbubble diameters and production rates is presented. A micro Coulter particle counter (μCPC), using electrodes integrated within the expanding nozzle of an FFMD (FFMD-μCPC), was designed, fabricated and tested. Finite element analysis (FEA) of optimal electrode geometry was performed and validated with experimental data. Electrical data was collected for 8-20 μm diameter microbubbles at production rates up to 3.25 × 105 MB s-1 and compared to both high-speed microscopy data and FEA simulations. Within a valid operating regime, Coulter counts of microbubble production rates matched optical reference values. The Coulter method agreed with the optical reference method in evaluating the microbubble diameter to a coefficient of determination of R2 = 0.91.

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