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Jindal S, Pennock ND, Klug A, et al. S-nitrosylated and non-nitrosylated COX2 have differential expression and distinct subcellular localization in normal and breast cancer tissue. NPJ Breast Cancer. 2020;6(1):62doi: 10.1038/s41523-020-00204-6.
Jindal, S., Pennock, N. D., Klug, A., Narasimhan, J., Calhoun, A., Roberts, M. R., Tamimi, R. M., Eliassen, A. H., Weinmann, S., Borges, V. F., & Schedin, P. (2020). S-nitrosylated and non-nitrosylated COX2 have differential expression and distinct subcellular localization in normal and breast cancer tissue. NPJ breast cancer, 6(1), 62. https://doi.org/10.1038/s41523-020-00204-6
Jindal, Sonali, et al. "S-nitrosylated and non-nitrosylated COX2 have differential expression and distinct subcellular localization in normal and breast cancer tissue." NPJ breast cancer vol. 6,1 (2020): 62. doi: https://doi.org/10.1038/s41523-020-00204-6
Jindal S, Pennock ND, Klug A, Narasimhan J, Calhoun A, Roberts MR, Tamimi RM, Eliassen AH, Weinmann S, Borges VF, Schedin P. S-nitrosylated and non-nitrosylated COX2 have differential expression and distinct subcellular localization in normal and breast cancer tissue. NPJ Breast Cancer. 2020 Nov 24;6(1):62. doi: 10.1038/s41523-020-00204-6. PMID: 33298921; PMCID: PMC7686348.
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NPJ Breast Cancer. 2020 Nov 24;6(1):62. doi: 10.1038/s41523-020-00204-6.

S-nitrosylated and non-nitrosylated COX2 have differential expression and distinct subcellular localization in normal and breast cancer tissue.

NPJ breast cancer

Sonali Jindal, Nathan D Pennock, Alex Klug, Jayasri Narasimhan, Andrea Calhoun, Michelle R Roberts, Rulla M Tamimi, A Heather Eliassen, Sheila Weinmann, Virginia F Borges, Pepper Schedin

Affiliations

  1. Department of Cell, Developmental & Cancer Biology, Oregon Health & Science University, 2720 SW Moody Ave., Mailing Code: KR-CDCB, Portland, OR, 97201, USA.
  2. Knight Cancer Institute, Oregon Health & Science University, 2720 SW Moody Ave., Mailing Code: KR-ADM, Portland, OR, 97201, USA.
  3. Department of Dermatology, Massachusetts General Hospital, 50 Staniford Street, Suite 200, Boston, MA, 02114, USA.
  4. Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.
  5. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, 02114, USA.
  6. Center for Health Research, Kaiser Permanente Northwest, 3800 N. Interstate Ave., Portland, OR, 97227, USA.
  7. School of Medicine, Division of Medical Oncology, University of Colorado Anschutz Medical Campus, MS8117, RC-1S, 8401K, 12801 E 17th Ave., Aurora, CO, 80045, USA.
  8. Young Women's Breast Cancer Translational Program, School of Medicine, Division of Medical Oncology, University of Colorado Anschutz Medical Campus, MS8117, RC-1S, 8401K, 12801 E 17th Ave., Aurora, CO, 80045, USA.
  9. Department of Cell, Developmental & Cancer Biology, Oregon Health & Science University, 2720 SW Moody Ave., Mailing Code: KR-CDCB, Portland, OR, 97201, USA. [email protected].
  10. Knight Cancer Institute, Oregon Health & Science University, 2720 SW Moody Ave., Mailing Code: KR-ADM, Portland, OR, 97201, USA. [email protected].
  11. School of Medicine, Division of Medical Oncology, University of Colorado Anschutz Medical Campus, MS8117, RC-1S, 8401K, 12801 E 17th Ave., Aurora, CO, 80045, USA. [email protected].
  12. Young Women's Breast Cancer Translational Program, School of Medicine, Division of Medical Oncology, University of Colorado Anschutz Medical Campus, MS8117, RC-1S, 8401K, 12801 E 17th Ave., Aurora, CO, 80045, USA. [email protected].

PMID: 33298921 PMCID: PMC7686348 DOI: 10.1038/s41523-020-00204-6

Abstract

Immunohistochemical (IHC) staining in breast cancer shows both gain and loss of COX2 expression with disease risk and progression. We investigated four common COX2 antibody clones and found high specificity for purified human COX2 for three clones; however, recognition of COX2 in cell lysates was clone dependent. Biochemical characterization revealed two distinct forms of COX2, with SP21 recognizing an S-nitrosylated form, and CX229 and CX294 recognizing non-nitrosylated COX2 antigen. We found S-nitrosylated and non-nitrosylated COX2 occupy different subcellular locations in normal and breast cancer tissue, implicating distinct synthetic/trafficking pathways and function. Dual stains of ~2000 breast cancer cases show early-onset breast cancer had increased expression of both forms of COX2 compared to postmenopausal cases. Our results highlight the strengths of using multiple, highly characterized antibody clones for COX2 IHC studies and raise the prospect that S-nitrosylation of COX2 may play a role in breast cancer biology.

References

  1. Carcinogenesis. 2009 Mar;30(3):377-86 - PubMed
  2. Asian Pac J Cancer Prev. 2018 Jul 27;19(7):1971-1975 - PubMed
  3. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1420-4 - PubMed
  4. Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10537-40 - PubMed
  5. Breast Cancer Res. 2015 Feb 22;17:25 - PubMed
  6. Gynecol Endocrinol. 2017 May;33(5):353-358 - PubMed
  7. Cancer Res. 2003 May 15;63(10):2347-50 - PubMed
  8. N Engl J Med. 2003 Mar 6;348(10):891-9 - PubMed
  9. Carcinogenesis. 2013 Mar;34(3):503-12 - PubMed
  10. Cancer Res. 2002 Jan 1;62(1):28-32 - PubMed
  11. N Engl J Med. 2006 Aug 31;355(9):885-95 - PubMed
  12. Ann Intern Med. 2013 Jul 16;159(2):77-85 - PubMed
  13. Eur J Surg Oncol. 2003 Nov;29(9):707-10 - PubMed
  14. J Pathol. 2006 Feb;208(3):356-63 - PubMed
  15. J Clin Oncol. 2005 Apr 20;23(12):2840-55 - PubMed
  16. Iran J Pharm Res. 2011 Fall;10(4):655-83 - PubMed
  17. Am J Public Health. 2016 Sep;106(9):1592-8 - PubMed
  18. Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8838-43 - PubMed
  19. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):13591-13593 - PubMed
  20. Cell. 1995 Nov 3;83(3):493-501 - PubMed
  21. Clin Cancer Res. 2008 Dec 15;14(24):8221-7 - PubMed
  22. Bioinformatics. 2001 Apr;17(4):309-18 - PubMed
  23. Am J Physiol Heart Circ Physiol. 2006 May;290(5):H1960-8 - PubMed
  24. Br J Cancer. 2004 Jan 26;90(2):423-9 - PubMed
  25. Cancer Res. 2002 Feb 1;62(3):632-5 - PubMed
  26. Br J Pharmacol. 2019 Jan;176(2):155-176 - PubMed
  27. J Biol Chem. 2001 May 25;276(21):18563-9 - PubMed
  28. Nat Med. 2011 Aug 07;17(9):1109-15 - PubMed
  29. Histopathology. 2004 Jul;45(1):47-54 - PubMed
  30. N Engl J Med. 2018 Oct 18;379(16):1519-1528 - PubMed
  31. J Natl Cancer Inst. 2008 Mar 19;100(6):421-7 - PubMed
  32. Oncol Rep. 2000 Nov-Dec;7(6):1377-81 - PubMed
  33. J Natl Cancer Inst. 2016 Aug 16;108(12): - PubMed
  34. Cancer Cell. 2004 Sep;6(3):285-95 - PubMed
  35. N Engl J Med. 2007 May 24;356(21):2131-42 - PubMed
  36. Breast Cancer Res Treat. 2011 Nov;130(2):657-62 - PubMed
  37. Traffic. 2018 May 16;: - PubMed
  38. Science. 2005 Dec 23;310(5756):1966-70 - PubMed
  39. Chin Med J (Engl). 2004 Aug;117(8):1151-4 - PubMed
  40. Ann Intern Med. 2015 Sep 1;163(5):347-55 - PubMed
  41. J Biol Chem. 2000 Oct 27;275(43):33951-6 - PubMed
  42. Trends Immunol. 2015 Mar;36(3):161-78 - PubMed
  43. J Natl Cancer Inst. 2002 Feb 20;94(4):252-66 - PubMed
  44. Clin Cancer Res. 2018 Mar 1;24(5):1152-1162 - PubMed
  45. Cancer Prev Res (Phila). 2009 Apr;2(4):310-21 - PubMed
  46. JAMA. 2005 Jul 6;294(1):47-55 - PubMed
  47. Mol Carcinog. 2003 May;37(1):25-31 - PubMed
  48. Cancer Res. 2002 Mar 15;62(6):1676-81 - PubMed
  49. Proc Natl Acad Sci U S A. 2003 May 13;100(10):5974-9 - PubMed
  50. Acta Histochem. 2016 Mar;118(2):176-82 - PubMed
  51. Cancer Res. 2000 Sep 15;60(18):5040-4 - PubMed
  52. FASEB J. 2001 Dec;15(14):2742-4 - PubMed
  53. NPJ Breast Cancer. 2020 Oct 16;6:55 - PubMed
  54. Am J Pathol. 2014 Apr;184(4):1219-1229 - PubMed
  55. Cancer. 2000 Dec 15;89(12):2637-45 - PubMed
  56. BMC Cancer. 2006 Jan 30;6:27 - PubMed
  57. J Natl Cancer Inst. 2010 May 5;102(9):627-37 - PubMed
  58. J Immunol. 1987 Jul 15;139(2):518-25 - PubMed
  59. Breast Cancer Res. 2017 May 1;19(1):52 - PubMed
  60. Br J Cancer. 2012 Oct 23;107(9):1602-7 - PubMed
  61. Mod Pathol. 2002 Dec;15(12):1318-25 - PubMed
  62. Int J Cancer. 2017 Jan 1;140(1):163-175 - PubMed
  63. Br J Cancer. 2003 Feb 24;88(4):574-8 - PubMed
  64. Cell. 2015 Sep 10;162(6):1257-70 - PubMed
  65. Cancer Res. 2008 Jan 15;68(2):467-75 - PubMed

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