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Vanloon J, Harroun T, Yan H. Circular dichroism spectroscopy of DNA duplexes at near-biological concentrations. Bioorg Med Chem Lett. 2021;43:128053doi: 10.1016/j.bmcl.2021.128053.
Vanloon, J., Harroun, T., & Yan, H. (2021). Circular dichroism spectroscopy of DNA duplexes at near-biological concentrations. Bioorganic & medicinal chemistry letters, 43128053. https://doi.org/10.1016/j.bmcl.2021.128053
Vanloon, Jesse, et al. "Circular dichroism spectroscopy of DNA duplexes at near-biological concentrations." Bioorganic & medicinal chemistry letters vol. 43 (2021): 128053. doi: https://doi.org/10.1016/j.bmcl.2021.128053
Vanloon J, Harroun T, Yan H. Circular dichroism spectroscopy of DNA duplexes at near-biological concentrations. Bioorg Med Chem Lett. 2021 Jul 01;43:128053. doi: 10.1016/j.bmcl.2021.128053. Epub 2021 Apr 22. PMID: 33895278.
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Bioorg Med Chem Lett. 2021 Jul 01;43:128053. doi: 10.1016/j.bmcl.2021.128053. Epub 2021 Apr 22.

Circular dichroism spectroscopy of DNA duplexes at near-biological concentrations.

Bioorganic & medicinal chemistry letters

Jesse Vanloon, Thad Harroun, Hongbin Yan

Affiliations

  1. Centre for Biotechnology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines L2S 3A1, Ontario, Canada.
  2. Department of Physics, Brock University, 1812 Sir Isaac Brock Way, St. Catharines L2S 3A1, Ontario, Canada. Electronic address: [email protected].
  3. Centre for Biotechnology, Brock University, 1812 Sir Isaac Brock Way, St. Catharines L2S 3A1, Ontario, Canada; Department of Chemistry, Brock University, 1812 Sir Isaac Brock Way, St. Catharines L2S 3A1, Ontario, Canada. Electronic address: [email protected].

PMID: 33895278 DOI: 10.1016/j.bmcl.2021.128053

Abstract

Circular dichroism (CD) of nucleic acids has been typically carried out at sample concentrations below 10 μM, which is far lower than nucleic acid concentrations in biological systems. Attempts to study nucleic acid conformations by CD at higher concentrations using 10 and 1 mm pathlength cuvettes led to instrument artifacts. By shortening the light pathlength to around 0.1 mm, we herein report the first CD profiles of nucleic acids at sub-mM concentrations, which are relevant to nucleic acid concentrations in cellular cytoplasm and nucleus. These CD experimental conditions will allow future conformational studies of nucleic acids under biologically relevant conditions.

Copyright © 2021 Elsevier Ltd. All rights reserved.

Keywords: Circular dichroism; Conformation; Nucleic acids; Pathlength

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