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Yu X, Chen R, Wang F, et al. Pattern recognition receptor-initiated innate immune responses in mouse prostatic epithelial cells‡. Biol Reprod. 2021;105(1):113-127doi: 10.1093/biolre/ioab076.
Yu, X., Chen, R., Wang, F., Liu, W., Zhang, W., Gong, M., Wu, H., Liu, A., Han, R., Chen, Y., & Han, D. (2021). Pattern recognition receptor-initiated innate immune responses in mouse prostatic epithelial cells‡. Biology of reproduction, 105(1), 113-127. https://doi.org/10.1093/biolre/ioab076
Yu, Xiaoqin, et al. "Pattern recognition receptor-initiated innate immune responses in mouse prostatic epithelial cells‡." Biology of reproduction vol. 105,1 (2021): 113-127. doi: https://doi.org/10.1093/biolre/ioab076
Yu X, Chen R, Wang F, Liu W, Zhang W, Gong M, Wu H, Liu A, Han R, Chen Y, Han D. Pattern recognition receptor-initiated innate immune responses in mouse prostatic epithelial cells‡. Biol Reprod. 2021 Jul 02;105(1):113-127. doi: 10.1093/biolre/ioab076. PMID: 33899078.
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Biol Reprod. 2021 Jul 02;105(1):113-127. doi: 10.1093/biolre/ioab076.

Pattern recognition receptor-initiated innate immune responses in mouse prostatic epithelial cells‡.

Biology of reproduction

Xiaoqin Yu, Ran Chen, Fei Wang, Weihua Liu, Wenjing Zhang, Maolei Gong, Han Wu, Aijie Liu, Ruiqin Han, Yongmei Chen, Daishu Han

Affiliations

  1. School of Basic Medicine, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
  2. Department of Immunology, Shenzhen University School of Medicine, Shenzhen, China.

PMID: 33899078 DOI: 10.1093/biolre/ioab076

Abstract

Three major pathogenic states of the prostate, including benign prostatic hyperplasia, prostate cancer, and prostatitis, are related to the local inflammation. However, the mechanisms underlying the initiation of prostate inflammation remain largely unknown. Given that the innate immune responses of the tissue-specific cells to microbial infection or autoantigens contribute to local inflammation, this study focused on pattern recognition receptor (PRR)-initiated innate immune responses in mouse prostatic epithelial cells (PECs). Primary mouse PECs abundantly expressed Toll-like receptor 3 (TLR3), TLR4, TLR5, melanoma differentiation-associated protein 5 (MDA5), and IFN-inducible protein 16 (p204 in mouse). These PRRs can be activated by their respective ligands: lipopolysaccharide (LPS) and flagellin of Gram-negative bacteria for TLR4 and TLR5, polyinosinic-polycytidylic acid (poly(I:C)) for TLR3 and MDA5, and herpes simplex virus DNA analog (HSV60) for p204. LPS and flagellin predominantly induced the expression of inflammatory cytokines, including tumor necrosis factor alpha (TNFA), interleukin 6 (IL6), chemokines monocyte chemoattractant protein-1 (MCP1), and C-X-C motif chemokine 10 (CXCL10). Poly(I:C) and HSV60 predominantly induced the expression of type 1 interferons (IFNA and IFNB) and antiviral proteins: Mx GTPase 1, 2',5'-oligoadenylate synthetase 1, and IFN-stimulated gene 15. The replication of mumps virus in PECs was inhibited by type 1 IFN signaling. These findings provide insights into the mechanisms underlying innate immune response in the prostate.

© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: [email protected].

Keywords: innate immune response; microbial infection; pattern recognition receptor; prostatic epithelial cell; prostatitis

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