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Ma S, Zheng L, Lin X, et al. Network Pharmacology and Metabolomics Studies on Antimigraine Mechanisms of Da Chuan Xiong Fang (DCXF). Evid Based Complement Alternat Med. 2021;2021:6665137doi: 10.1155/2021/6665137.
Ma, S., Zheng, L., Lin, X., Feng, Y., Yang, M., & Shen, L. (2021). Network Pharmacology and Metabolomics Studies on Antimigraine Mechanisms of Da Chuan Xiong Fang (DCXF). Evidence-based complementary and alternative medicine : eCAM, 20216665137. https://doi.org/10.1155/2021/6665137
Ma, Shiyu, et al. "Network Pharmacology and Metabolomics Studies on Antimigraine Mechanisms of Da Chuan Xiong Fang (DCXF)." Evidence-based complementary and alternative medicine : eCAM vol. 2021 (2021): 6665137. doi: https://doi.org/10.1155/2021/6665137
Ma S, Zheng L, Lin X, Feng Y, Yang M, Shen L. Network Pharmacology and Metabolomics Studies on Antimigraine Mechanisms of Da Chuan Xiong Fang (DCXF). Evid Based Complement Alternat Med. 2021 Apr 20;2021:6665137. doi: 10.1155/2021/6665137. eCollection 2021. PMID: 33995549; PMCID: PMC8081595.
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Evid Based Complement Alternat Med. 2021 Apr 20;2021:6665137. doi: 10.1155/2021/6665137. eCollection 2021.

Network Pharmacology and Metabolomics Studies on Antimigraine Mechanisms of Da Chuan Xiong Fang (DCXF).

Evidence-based complementary and alternative medicine : eCAM

Shiyu Ma, Lin Zheng, Xiao Lin, Yi Feng, Ming Yang, Lan Shen

Affiliations

  1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  2. Department of Pharmacy, Ruijin Hospital, Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
  3. Department of Bone and Joint Surgery, Shanghai GuangHua Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, China.
  4. Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of the Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  5. Department of Good Clinical Practice, Longhua Hospital, Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

PMID: 33995549 PMCID: PMC8081595 DOI: 10.1155/2021/6665137

Abstract

BACKGROUND: Da Chuan Xiong Fang (DCXF) is a traditional Chinese medicine (TCM) formula used to treat migraines. Previously, we uncovered partial mechanisms involved in the therapeutic actions of DCXF on migraines.

METHODS: In this study, we further elucidated its antimigraine mechanisms in vivo by using an integrated strategy coupling with network pharmacology and metabolomics techniques.

RESULTS: Network pharmacology identified 33 genes linked with both migraine and DCXF, most of which were 5-hydroxytryptamine receptors, dopamine, and peptide receptors. The results of GO and KEGG enrichment analysis showed that DCXF significantly regulated tyrosine metabolism, tryptophan metabolism, dopamine metabolic process, glucose transmembrane transport, lipid metabolism, and fatty acid transport. The results of metabolomics analysis found that the metabolism of tryptophan and tyrosine in the brain tissue and energy and lipid metabolism of rats tended towards normal and reached normal levels after administering DCXF. The metabolomics and network pharmacology approaches demonstrated similar antimigraine effects of DCXF on endogenous neurotransmitters and overall trends in serum and brain tissue. Using both approaches, 62 hub genes were identified from the protein-protein interaction (PPI) network of DCXF and gene-metabolite interaction network, with hub genes and different metabolites in serum and brain tissue. The hub genes of DCXF, which were mostly linked with inflammation, might affect mainly neurotransmitters in serum and brain tissue metabolisms.

CONCLUSION: Network pharmacology and metabolomics study may help identify hub genes, metabolites, and possible pathways of disease and treatment. Additionally, two parts of the results were integrated to confirm each other. Their combination may help elucidate the relationship between hub genes and metabolites and provide the further understanding of TCM mechanisms.

Copyright © 2021 Shiyu Ma et al.

Conflict of interest statement

The authors declare that they have no conflicts of interest.

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