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Luo XY, Zhao X, Ren L, et al. [Study on the Analytical Method of Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Quadrupole Linear Ion Trap Mass Spectrometry for 12 Perfluorinated Compounds in Human Urine]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2021;52(4):679-685doi: 10.12182/20210760504.
Luo, X. Y., Zhao, X., Ren, L., Wang, W. J., Yong, L., Yuan, Y., Zeng, H. Y., & Zou, X. L. (2021). [Study on the Analytical Method of Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Quadrupole Linear Ion Trap Mass Spectrometry for 12 Perfluorinated Compounds in Human Urine]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 52(4), 679-685. https://doi.org/10.12182/20210760504
Luo, Xin-Yue, et al. "[Study on the Analytical Method of Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Quadrupole Linear Ion Trap Mass Spectrometry for 12 Perfluorinated Compounds in Human Urine]." Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition vol. 52,4 (2021): 679-685. doi: https://doi.org/10.12182/20210760504
Luo XY, Zhao X, Ren L, Wang WJ, Yong L, Yuan Y, Zeng HY, Zou XL. [Study on the Analytical Method of Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Quadrupole Linear Ion Trap Mass Spectrometry for 12 Perfluorinated Compounds in Human Urine]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2021 Jul;52(4):679-685. doi: 10.12182/20210760504. Chinese. PMID: 34323049.
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Sichuan Da Xue Xue Bao Yi Xue Ban. 2021 Jul;52(4):679-685. doi: 10.12182/20210760504.

[Study on the Analytical Method of Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Quadrupole Linear Ion Trap Mass Spectrometry for 12 Perfluorinated Compounds in Human Urine].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition

[Article in Chinese]
Xin-Yue Luo, Xuan Zhao, Lin Ren, Wen-Jia Wang, Li Yong, Yue Yuan, Hong-Yan Zeng, Xiao-Li Zou

Affiliations

  1. Department of Laboratory Technology and Science of Public Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
  2. Sichuan Provincial Center for Disease Control and Prevention, Chengdu 610041, China.

PMID: 34323049 DOI: 10.12182/20210760504

Abstract

OBJECTIVE: To establish a method for simultaneous determination of 12 kinds of perfluorinated compounds (PFCs) in human urine based on ultra performance liquid chromatography tandem quadrupole linear ion trap mass spectrometry (UPLC-QTtrap-MS).

METHODS: After pH adjustment with 2% formic acid, the urine samples were loaded on a WAX solid phase extraction (SPE) cartridge for extraction, purification and concentration. The eluates were collected, concentrated to dryness under nitrogen, and reconstituted with 10 mmol/L ammonium acetate aqueous solution-methanol (

RESULTS: Good linearity was obtained in the linear range, with the method detection limits and method quantification limits being 0.032 ng/L-6.5 ng/L and 0.10 ng/L-21 ng/L, respectively, for the 12 kinds of PFCs. The spiked recoveries of the 12 kinds of PFCs were 91.5%-114%, with the intra-day precision and the inter-day precision being 0.57%-16.0% and 1.88%-20.1%, respectively. The established method was applied to the determination of 12 kinds of PFCs in the urine samples of primary school students collected in one area. Nine kinds of PFCs were detected in the urine samples in this area. Among the PFCs detected, perfluorobutanesulfonic acid (PFBS) and perfluorooctanoic acid (PFOA) were the main PFCs found in the student urine samples.

CONCLUSION: The method established in this study could be used to simultaneously examine 12 kinds of PFCs in urine. The method combined SPE with isotope internal standard correction and achieved good sensitivity and accuracy.

Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).

Keywords: Perfluorinated compounds; Solid phase extraction; UPLC-QTtrap-MS; Urine

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