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Lin C, Liu Z, Li Y, et al. Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration. STAR Protoc. 2021;2(3):100720doi: 10.1016/j.xpro.2021.100720.
Lin, C., Liu, Z., Li, Y., & Chen, J. (2021). Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration. STAR protocols, 2(3), 100720. https://doi.org/10.1016/j.xpro.2021.100720
Lin, ChangDong, et al. "Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration." STAR protocols vol. 2,3 (2021): 100720. doi: https://doi.org/10.1016/j.xpro.2021.100720
Lin C, Liu Z, Li Y, Chen J. Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration. STAR Protoc. 2021 Aug 05;2(3):100720. doi: 10.1016/j.xpro.2021.100720. eCollection 2021 Sep 17. PMID: 34401786; PMCID: PMC8353337.
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STAR Protoc. 2021 Aug 05;2(3):100720. doi: 10.1016/j.xpro.2021.100720. eCollection 2021 Sep 17.

Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration.

STAR protocols

ChangDong Lin, ZhaoYuan Liu, Yue Li, JianFeng Chen

Affiliations

  1. State Key Laboratory of Cell Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
  2. School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.

PMID: 34401786 PMCID: PMC8353337 DOI: 10.1016/j.xpro.2021.100720

Abstract

Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We describe the isolation of T cells from mouse spleen followed by the evaluation of T-cell adhesion and transmigration. This animal model can be applied to studying the dysfunction of the immune system induced by fever. For complete details on the use and execution of this protocol, please refer to Lin et al. (2019).

© 2021 The Author(s).

Keywords: Cell Biology; Cell isolation; Immunology; Model Organisms

Conflict of interest statement

The authors declare no competing interests.

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