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Klinkmann G, Doss F, Goudeva L, et al. Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method. Transfusion. 2021;62(1):194-204doi: 10.1111/trf.16732.
Klinkmann, G., Doss, F., Goudeva, L., Doss, S., Blasczyk, R., Milej, M., Koch, S., Mitzner, S., & Altrichter, J. (2022). Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method. Transfusion, 62(1), 194-204. https://doi.org/10.1111/trf.16732
Klinkmann, Gerd, et al. "Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method." Transfusion vol. 62,1 (2022): 194-204. doi: https://doi.org/10.1111/trf.16732
Klinkmann G, Doss F, Goudeva L, Doss S, Blasczyk R, Milej M, Koch S, Mitzner S, Altrichter J. Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method. Transfusion. 2022 Jan;62(1):194-204. doi: 10.1111/trf.16732. Epub 2021 Nov 16. PMID: 34783358.
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Transfusion. 2022 Jan;62(1):194-204. doi: 10.1111/trf.16732. Epub 2021 Nov 16.

Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method.

Transfusion

Gerd Klinkmann, Fanny Doss, Lilia Goudeva, Sandra Doss, Rainer Blasczyk, Magdalena Milej, Stephanie Koch, Steffen Mitzner, Jens Altrichter

Affiliations

  1. Department of Anaesthesiology and Intensive Care Medicine, University of Rostock, Rostock, Germany.
  2. Artcline GmbH, Rostock, Germany.
  3. Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany.
  4. Department of Extracorporeal Immunomodulation, Fraunhofer Institute for Cell Therapy and Immunology, Rostock, Germany.
  5. Department of Medicine, Division of Nephrology, Medical Faculty, University of Rostock, Rostock, Germany.

PMID: 34783358 DOI: 10.1111/trf.16732

Abstract

BACKGROUND: Use of donor granulocyte concentrate (GC) has been limited due to its short storage time of 6-24 h, which is partially due to residual red blood cells (RBCs) and platelets and the resulting lactate production leading to an acidotic milieu. To increase this storage time, we developed a closed system procedure compatible with standard blood bank technologies to remove RBC and platelets and to enrich the GC.

METHODS: Standard GCs (sGCs) were sedimented, washed twice with 0.9% sodium chloride (NaCl), and resuspended in blood group-identical fresh frozen plasma. The resulting purified GCs (pGCs) were then stored in platelet bags at a cell concentration of about 5 × 10

RESULTS: A significant reduction in RBC (98%) through sedimentation, and platelets (96%) by washing, purified the white blood cell (WBC) population and enriched the granulocytes to 96% of the WBC in the pGC. After 72 h of storage, over 90% of the initial WBC count of pGC remained, was viable (≥97%), and the granulocytes exhibited a high phagocytosis and oxidative burst functionality, comparable to sGC after 24 h.

CONCLUSION: Purification extends the maximum storage period of GC from 24 to 72 h and may therefore improve the availability of GC and its clinical use.

© 2021 The Authors. Transfusion published by Wiley Periodicals LLC on behalf of AABB.

Keywords: granulocyte; purification; transfusion; transfusion medicine

References

  1. Rudd KE, Johnson SJ, Agesa KM, Shackelford KA, Tsoi D, Kievlan DR, et al. Global, regional, and national sepsis incidence and mortality, 1990-2017: analysis for the Global Burden of Disease Study. Lancet. 2020;395:200-11. - PubMed
  2. Yeghen T, Devereux S. Granulocyte transfusion: a review. Vox Sang. 2001;81:87-92. - PubMed
  3. Summers C, Rankin SM, Condliffe AM, Singh N, Peters AM, Chilvers ER. Neutrophil kinetics in health and disease. Trends Immunol. 2010;31:318-24. - PubMed
  4. Brostjan C, Oehler R. The role of neutrophil death in chronic inflammation and cancer. Cell Death Discov. 2020;6:26. - PubMed
  5. Boomer JS, To K, Chang KC, Takasu O, Osborne DF, Walton AH, et al. Immunosuppression in patients who die of sepsis and multiple organ failure. JAMA. 2011;306:2594-605. - PubMed
  6. Frazier WJ, Hall MW. Immunoparalysis and adverse outcomes from critical illness. Pediatr Clin North Am. 2008;55:647-xi. - PubMed
  7. Kaufmann I, Hoelzl A, Schliephake F, Hummel T, Chouker A, Peter K, et al. Polymorphonuclear leukocyte dysfunction syndrome in patients with increasing sepsis severity. Shock. 2006;26:254-61. - PubMed
  8. Tavares-Murta BM, Zaparoli M, Ferreira RB, Silva-Vergara ML, Oliveira CHB, Murta EFC, et al. Failure of neutrophil chemotactic function in septic patients. Crit Care Med. 2002;30:1056-61. - PubMed
  9. Keel M, Ungethüm U, Steckholzer U, Niederer E, Hartung T, Trentz O, et al. Interleukin-10 counterregulates proinflammatory cytokine-induced inhibition of neutrophil apoptosis during severe sepsis. Blood. 1997;90:3356-63. - PubMed
  10. Egger G, Aigner R, Glasner A, Hofer HP, Mitterhammer H, Zelzer S. Blood polymorphonuclear leukocyte migration as a predictive marker for infections in severe trauma: comparison with various inflammation parameters. Intensive Care Med. 2004;30:331-4. - PubMed
  11. Wenisch C, Parschalk P, Hasenhündl M, Griesmacher A, Graninger W. Polymorphonuclear leucocyte dysregulation in patients with gram-negative septicaemia assessed by flow cytometry. Eur J Clin Invest. 1995;25:418-24. - PubMed
  12. Lehmann LE, Novender U, Schroeder S, Pietsch T, von Spiegel T, Putensen C, et al. Plasma levels of macrophage migration inhibitory factor are elevated in patients with severe sepsis. Intensive Care Med. 2001;27:1412-5. - PubMed
  13. Caille V, Chiche J-D, Nciri N, Berton C, Gibot S, Boval B, et al. Histocompatibility leukocyte antigen-D related expression is specifically altered and predicts mortality in septic shock but not in other causes of shock. Shock. 2004;22:521-6. - PubMed
  14. Pachot A, Monneret G, Brion A, Venet F, Bohé J, Bienvenu J, et al. Messenger RNA expression of major histocompatibility complex class II genes in whole blood from septic shock patients. Crit Care Med. 2005;33:31-8. - PubMed
  15. Hotchkiss RS, Tinsley KW, Swanson PE, Schmieg RE Jr, Hui JJ, Chang KC, et al. Sepsis-induced apoptosis causes progressive profound depletion of B and CD4+ T lymphocytes in humans. J Immunol. 2001;166:6952-63. - PubMed
  16. Delano MJ, Ward PA. Sepsis-induced immune dysfunction: can immune therapies reduce mortality? J Clin Invest. 2016;126(1):23-31. - PubMed
  17. Freireich EJ, Levin RH, Whang J, Carbone PP, Bronson W, Morse EE. The function and fate of transfused leukocytes from donors with chronic myelocytic leukemia to leukopenic recipients. Ann N Y Acad Sci. 1964;113:1081-9. - PubMed
  18. Morse EE, Freireich EJ, Carbone PP, Bronson W, Frei E. The transfusion of leukocytes from donors with myelocytic leukemia to patients with leukopenia. Transfusion. 1966;6:183-92. - PubMed
  19. Van Burik JA, Weisdorf DJ. Is it time for a new look at granulocyte transfusions? Transfusion. 2002;42:1393-5. - PubMed
  20. Price TH. The RING study: a randomized controlled trial of GCSF-stimulated granulocytes in granulocytopenic patients. Blood. 2014;124:21-16. - PubMed
  21. Altrichter J, Sauer M, Kaftan K, Birken T, Gloger D, Gloger M, et al. Extracorporeal cell therapy of septic shock patients with donor granulocytes: a pilot study. Crit Care. 2011;15:R82. - PubMed
  22. AABB. Circular of information for the use of blood and blood components 2017, p. 46. Available from: https://www.aabb.org/docs/default-source/default-document-library/resources/circular-of-information-10-17.pdf?sfvrsn=7d348f7b_0. Accessed 13 Apr 2021. - PubMed
  23. Selders GS, Fetz AE, Radic MZ, Bowlin GL. An overview of the role of neutrophils in innate immunity, inflammation and host-biomaterial integration. Regen Biomater. 2017;4:55-68. - PubMed
  24. Bashir S, Cardigan R. Granulocyte concentrates: how can we assess their quality? Transfus Med. 2003;13:245-57. - PubMed
  25. Price TH, Boeckh M, Harrison RW, McCullough J, Ness PM, Strauss RG, et al. Efficacy of transfusion with granulocytes from G-CSF/dexamethasone-treated donors in neutropenic patients with infection. Blood. 2015;126:2153-61. - PubMed
  26. Council of Europe. Guide to the preparation, use and quality assurance of blood components, The European Directorate for the Quality of Medicines & HealthCare; 2020. - PubMed
  27. Yoshida T, Prudent M, D'Alessandro A. Red blood cell storage lesion: causes and potential clinical consequences. Blood Transfus. 2019;17:27-52. - PubMed
  28. Keating FK, Butenas S, Fung MK, Schneider DJ. Platelet-white blood cell (WBC) interaction, WBC apoptosis, and procoagulant activity in stored red blood cells. Transfusion. 2011;51:1086-95. - PubMed
  29. German Medical Association (Bundesärztekammer). Cross-sectional guidelines for therapy with blood components and plasma derivatives - general amendment 2020 (Querschnitts-Leitlinien zur Therapie mit Blutkomponenten und Plasmaderivaten, Gesamtnovelle 2020: in der vom Vorstand der Bundesärztekammer auf Empfehlung des Wissenschaftlichen Beirats am 21.08.2020 beschlossenen Fassung). - PubMed
  30. World Health Organization. Definition and diagnosis of diabetes mellitus and intermediate hyperglycaemia. 2006 Jan 14. Available from: https://www.who.int/publications/i/item/definition-and-diagnosis-of-diabetes-mellitus-and-intermediate-hyperglycaemia. Accessed 2 Jun 2021. - PubMed
  31. Dullinger K, Pamler I, Brosig A, Mohrez M, Hähnel V, Offner R, et al. Granulocytapheresis with modifed fluid gelatin versus high-molecular-weight hydroxyethyl starch: a matched-pair analysis. Transfusion. 2017;57:397-403. - PubMed
  32. Glasser L, Fiederlein RL, Huestis DW. Granulocyte concentrates: glucose concentrations and glucose utilization during storage at 22°C. Transfusion. 1985;25:68-9. - PubMed
  33. Bashir S, Stanworth S, Massey E, Goddard F, Cardigan R. Neutrophil function is preserved in a pooled granulocyte component prepared from whole blood donations. Br J Haematol. 2008;140:701-11. - PubMed
  34. Bryant BJ, Yau YY, Byrne PJ, Stroncek DF, Leitman SF. Gravity sedimentation of granulocytapheresis concentrates with hydroxyethyl starch efficiently removes red blood cells and retains neutrophils. Transfusion. 2010;50:1203-9. - PubMed
  35. Valerius NH, Johansen KS, Nielsen OS, Platz P, Rosenkvist J, Sørensen H. Effect of in vitro X-irradiation on lymphocyte and granulocyte function. Scand J Haematol. 1981;27:9-18. - PubMed
  36. De Vries A, Holzberger P, Kunc M, Hengster P. Influence of irradiation on Neutrophilic granulocyte function. Cancer. 2001;92:2444-50. - PubMed
  37. Haidenberger A, Hengster P, Kunc M, Micke O, Wolfgruber T, Auer T, et al. Influence of fractionated irradiation on neutrophilic granulocyte function. Strahlenther Onkol. 2003;179:45-9. - PubMed
  38. Buescher ES, Gallin JI. Effects of storage and radiation on human neutrophil function in vitro. Inflammation. 1987;11:401-16. - PubMed

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