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Liu J, Li Y, Pu Q, et al. A polysaccharide from Lycium barbarum L.: Structure and protective effects against oxidative stress and high-glucose-induced apoptosis in ARPE-19 cells. Int J Biol Macromol. 2021;201:111-120doi: 10.1016/j.ijbiomac.2021.12.139.
Liu, J., Li, Y., Pu, Q., Qiu, H., Di, D., & Cao, Y. (2021). A polysaccharide from Lycium barbarum L.: Structure and protective effects against oxidative stress and high-glucose-induced apoptosis in ARPE-19 cells. International journal of biological macromolecules, 201111-120. https://doi.org/10.1016/j.ijbiomac.2021.12.139
Liu, Jianfei, et al. "A polysaccharide from Lycium barbarum L.: Structure and protective effects against oxidative stress and high-glucose-induced apoptosis in ARPE-19 cells." International journal of biological macromolecules vol. 201 (2021): 111-120. doi: https://doi.org/10.1016/j.ijbiomac.2021.12.139
Liu J, Li Y, Pu Q, Qiu H, Di D, Cao Y. A polysaccharide from Lycium barbarum L.: Structure and protective effects against oxidative stress and high-glucose-induced apoptosis in ARPE-19 cells. Int J Biol Macromol. 2021 Dec 27;201:111-120. doi: 10.1016/j.ijbiomac.2021.12.139. Epub 2021 Dec 27. PMID: 34968548.
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Int J Biol Macromol. 2021 Dec 27;201:111-120. doi: 10.1016/j.ijbiomac.2021.12.139. Epub 2021 Dec 27.

A polysaccharide from Lycium barbarum L.: Structure and protective effects against oxidative stress and high-glucose-induced apoptosis in ARPE-19 cells.

International journal of biological macromolecules

Jianfei Liu, Yunchun Li, Qiaosheng Pu, Hongdeng Qiu, Duolong Di, Youlong Cao

Affiliations

  1. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  2. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
  3. State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
  4. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: [email protected].
  5. National Wolfberry Engineering Research Center, Yinchuan 750002, Ningxia, China. Electronic address: [email protected].

PMID: 34968548 DOI: 10.1016/j.ijbiomac.2021.12.139

Abstract

Lycium barbarum polysaccharides (LBPs) are beneficial for vision; however, relevant research has mainly focused on entire crude polysaccharides, with the basis and exact structure of the polysaccharide rarely explored. In this study, LICP009-3F-2a, a novel polysaccharide from Lycium barbarum L., was separated and then purified using anion-exchange and size-exclusion chromatography. Structural characteristics were investigated using chemical and spectroscopic methods, which revealed that LICP009-3F-2a has an Mw of 13720 Da and is an acidic heteropolysaccharide composed of rhamnose (39.1%), arabinose (7.4%), galactose (22.5%), glucose (8.3%), galacturonic acid (13.7%), and glucuronic acid (4.0%). Linkage and NMR data revealed that LICP009-3F-2a has the following backbone: →2)-α-L-Rha-(1 → 2,4)-α-L-Rha-(1 → 4)-α-D-GalAp-(1 → 3,6)-β-D-Galp-(1 → 3,6)-β-D-Galp-(1 → 6)-β-D-Galp-(1→, with three main branches, including: α-L-Araf-(1 → 5)-α-L-Araf-(1 → 6)-β-D-Glcp-(1 → 2,4)-α-L-Rha-(1→, β-D-Glcp-(1 → 4)-β-D-Glcp-(1 → 3,6)-β-D-Galp-(1→, and β-D-Galp-(1 → 3)-β-D-Galp-(1 → 3,6)-β-D-Galp-(1 → . Differential scanning colorimetry and thermogravimetric analysis showed that LICP009-3F-2a is thermally stable, while X-ray diffractometry showed that LICP009-3F-2a has a semi-crystalline structure. In addition, LICP009-3F-2a protects ARPE-19 cells from H

Copyright © 2021. Published by Elsevier B.V.

Keywords: ARPE-19 cells; Lycium barbarum polysaccharides; Structural characterization

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