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Kluza J, Peugnet V, Daunou B, et al. A New Strategy to Preserve and Assess Oxygen Consumption in Murine Tissues. Int J Mol Sci. 2021;23(1)doi: 10.3390/ijms23010109.
Kluza, J., Peugnet, V., Daunou, B., Laine, W., Kervoaze, G., Rémy, G., Loyens, A., Maboudou, P., Fovez, Q., Grangette, C., Wolowczuk, I., Gosset, P., Garçon, G., Marchetti, P., Pinet, F., Pichavant, M., & Dubois-Deruy, E. (2021). A New Strategy to Preserve and Assess Oxygen Consumption in Murine Tissues. International journal of molecular sciences, 23(1), . https://doi.org/10.3390/ijms23010109
Kluza, Jerome, et al. "A New Strategy to Preserve and Assess Oxygen Consumption in Murine Tissues." International journal of molecular sciences vol. 23,1 (2021). doi: https://doi.org/10.3390/ijms23010109
Kluza J, Peugnet V, Daunou B, Laine W, Kervoaze G, Rémy G, Loyens A, Maboudou P, Fovez Q, Grangette C, Wolowczuk I, Gosset P, Garçon G, Marchetti P, Pinet F, Pichavant M, Dubois-Deruy E. A New Strategy to Preserve and Assess Oxygen Consumption in Murine Tissues. Int J Mol Sci. 2021 Dec 22;23(1). doi: 10.3390/ijms23010109. PMID: 35008535; PMCID: PMC8745047.
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Int J Mol Sci. 2021 Dec 22;23(1). doi: 10.3390/ijms23010109.

A New Strategy to Preserve and Assess Oxygen Consumption in Murine Tissues.

International journal of molecular sciences

Jerome Kluza, Victoriane Peugnet, Blanche Daunou, William Laine, Gwenola Kervoaze, Gaëlle Rémy, Anne Loyens, Patrice Maboudou, Quentin Fovez, Corinne Grangette, Isabelle Wolowczuk, Philippe Gosset, Guillaume Garçon, Philippe Marchetti, Florence Pinet, Muriel Pichavant, Emilie Dubois-Deruy

Affiliations

  1. Univ. Lille, CNRS, Inserm, CHU Lille, Institut pour la Recherche sur le Cancer de Lille, UMR9020-UMR-S 1277-Canther-Cancer Heterogeneity, Plasticity and Resistance to Therapies, 59000 Lille, France.
  2. Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1167-RID-AGE-Facteurs de Risque et Déterminants Moléculaires des Maladies Liées au Vieillissement, 59000 Lille, France.
  3. Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Center for Infection and Immunity of Lille, 59000 Lille, France.
  4. Centre de Bio-Pathologie, CHRU Lille, 59000 Lille, France.
  5. Univ. Lille, CHU Lille, Institut Pasteur de Lille, ULR 4483-IMPacts de l'Environnement Chimique sur la Santé (IMPECS), 59000 Lille, France.

PMID: 35008535 PMCID: PMC8745047 DOI: 10.3390/ijms23010109

Abstract

Mitochondrial dysfunctions are implicated in several pathologies, such as metabolic, cardiovascular, respiratory, and neurological diseases, as well as in cancer and aging. These metabolic alterations are usually assessed in human or murine samples by mitochondrial respiratory chain enzymatic assays, by measuring the oxygen consumption of intact mitochondria isolated from tissues, or from cells obtained after physical or enzymatic disruption of the tissues. However, these methodologies do not maintain tissue multicellular organization and cell-cell interactions, known to influence mitochondrial metabolism. Here, we develop an optimal model to measure mitochondrial oxygen consumption in heart and lung tissue samples using the XF24 Extracellular Flux Analyzer (Seahorse) and discuss the advantages and limitations of this technological approach. Our results demonstrate that tissue organization, as well as mitochondrial ultrastructure and respiratory function, are preserved in heart and lung tissues freshly processed or after overnight conservation at 4 °C. Using this method, we confirmed the repeatedly reported obesity-associated mitochondrial dysfunction in the heart and extended it to the lungs. We set up and validated a new strategy to optimally assess mitochondrial function in murine tissues. As such, this method is of great potential interest for monitoring mitochondrial function in cohort samples.

Keywords: energy metabolism; high fat diet; oxidative phosphorylation; oximetry

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