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Jeffries CD, Fried HM, Perkins DO. Nuclear and cytoplasmic localization of neural stem cell microRNAs. RNA. 2011;17(4):675-86doi: 10.1261/rna.2006511.
Jeffries, C. D., Fried, H. M., & Perkins, D. O. (2011). Nuclear and cytoplasmic localization of neural stem cell microRNAs. RNA (New York, N.Y.), 17(4), 675-86. https://doi.org/10.1261/rna.2006511
Jeffries, Clark D, et al. "Nuclear and cytoplasmic localization of neural stem cell microRNAs." RNA (New York, N.Y.) vol. 17,4 (2011): 675-86. doi: https://doi.org/10.1261/rna.2006511
Jeffries CD, Fried HM, Perkins DO. Nuclear and cytoplasmic localization of neural stem cell microRNAs. RNA. 2011 Apr;17(4):675-86. doi: 10.1261/rna.2006511. Epub 2011 Mar 01. PMID: 21363885; PMCID: PMC3062178.
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RNA. 2011 Apr;17(4):675-86. doi: 10.1261/rna.2006511. Epub 2011 Mar 01.

Nuclear and cytoplasmic localization of neural stem cell microRNAs.

RNA (New York, N.Y.)

Clark D Jeffries, Howard M Fried, Diana O Perkins

Affiliations

  1. Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA. [email protected]

PMID: 21363885 PMCID: PMC3062178 DOI: 10.1261/rna.2006511

Abstract

Although generally regarded as functional in the cytoplasm, a number of microRNAs (miRNAs) have been found in the nucleus, possibly with a role in gene regulation. Here we report that, in fact, a substantial fraction of all human miRNAs are present in the nucleus of neural stem cells. Further, subsets of these miRNAs display consistently higher standardized rank in the nucleus than in the cytoplasm of these cells, as identified with an RT-qPCR technology and confirmed by microarray analysis. Likewise, other miRNAs display higher cytoplasmic standardized ranks. Three samples were partitioned into nuclear and cytoplasmic fractions in six assays for 373 miRNAs. From the 100 most highly expressed miRNAs, standard scores of nuclear and cytoplasmic concentrations were determined. Among those, 21 miRNAs had all three nuclear standard scores higher than all three cytoplasmic scores; likewise, 31 miRNAs had consistently higher cytoplasmic scores. Random concentrations would result in only five in each set. Remarkably, if one miRNA has a high standard score in a compartment, then other miRNAs having the same 5' seeds and certain similar 3' end patterns are also highly scored in the same way. That is, in addition to the seed sequence, 3' sequence similarity criteria identify families of mature miRNAs with consistently high nuclear or cytoplasmic expression.

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