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Li C, Zhao GQ, Che CY, et al. Expression of dectin-1 during fungus infection in human corneal epithelial cells. Int J Ophthalmol. 2014;7(1):34-7doi: 10.3980/j.issn.2222-3959.2014.01.06.
Li, C., Zhao, G. Q., Che, C. Y., Li, N., Lin, J., Xu, Q., Wang, Q., Liu, Y., & Qiu, S. (2014). Expression of dectin-1 during fungus infection in human corneal epithelial cells. International journal of ophthalmology, 7(1), 34-7. https://doi.org/10.3980/j.issn.2222-3959.2014.01.06
Li, Cui, et al. "Expression of dectin-1 during fungus infection in human corneal epithelial cells." International journal of ophthalmology vol. 7,1 (2014): 34-7. doi: https://doi.org/10.3980/j.issn.2222-3959.2014.01.06
Li C, Zhao GQ, Che CY, Li N, Lin J, Xu Q, Wang Q, Liu Y, Qiu S. Expression of dectin-1 during fungus infection in human corneal epithelial cells. Int J Ophthalmol. 2014 Feb 18;7(1):34-7. doi: 10.3980/j.issn.2222-3959.2014.01.06. eCollection 2014. PMID: 24634860; PMCID: PMC3949455.
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Int J Ophthalmol. 2014 Feb 18;7(1):34-7. doi: 10.3980/j.issn.2222-3959.2014.01.06. eCollection 2014.

Expression of dectin-1 during fungus infection in human corneal epithelial cells.

International journal of ophthalmology

Cui Li, Gui-Qiu Zhao, Cheng-Ye Che, Na Li, Jing Lin, Qiang Xu, Qian Wang, Ying Liu, Sheng Qiu

Affiliations

  1. Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China.

PMID: 24634860 PMCID: PMC3949455 DOI: 10.3980/j.issn.2222-3959.2014.01.06

Abstract

AIM: To evaluate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus.

METHODS: A total of 20 cases of healthy donor corneas were group A, and 20 patients (20 eyes) suffered from fungal keratitis (FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus (AF) antigens in vitro. The expression of dectin-1 mRNA was measured by real-time qPCR at the stimulation of 0, 4, 8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately.

RESULTS: Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin-1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h, and dectin-1 protein expression increased after stimulation at 24h.

CONCLUSION: Dectin-1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin-1 in HCE cells in vitro.

Keywords: corneal epithelial cells; dectin-1; fungal keratitis; human

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