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Deng Y, Li Y, Sun H. Selection of reference genes for RT-qPCR normalization in blueberry (Vaccinium corymbosum × angustifolium) under various abiotic stresses. FEBS Open Bio. 2020;10(8):1418-1435doi: 10.1002/2211-5463.12903.
Deng, Y., Li, Y., & Sun, H. (2020). Selection of reference genes for RT-qPCR normalization in blueberry (Vaccinium corymbosum × angustifolium) under various abiotic stresses. FEBS open bio, 10(8), 1418-1435. https://doi.org/10.1002/2211-5463.12903
Deng, Yu, et al. "Selection of reference genes for RT-qPCR normalization in blueberry (Vaccinium corymbosum × angustifolium) under various abiotic stresses." FEBS open bio vol. 10,8 (2020): 1418-1435. doi: https://doi.org/10.1002/2211-5463.12903
Deng Y, Li Y, Sun H. Selection of reference genes for RT-qPCR normalization in blueberry (Vaccinium corymbosum × angustifolium) under various abiotic stresses. FEBS Open Bio. 2020 Aug;10(8):1418-1435. doi: 10.1002/2211-5463.12903. Epub 2020 Jun 23. PMID: 32438500; PMCID: PMC7396441.
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FEBS Open Bio. 2020 Aug;10(8):1418-1435. doi: 10.1002/2211-5463.12903. Epub 2020 Jun 23.

Selection of reference genes for RT-qPCR normalization in blueberry (Vaccinium corymbosum × angustifolium) under various abiotic stresses.

FEBS open bio

Yu Deng, Yadong Li, Haiyue Sun

Affiliations

  1. College of Life Sciences, Jilin Agricultural University, Changchun, China.
  2. Engineering Center of Genetic Breeding and Innovative Utilization of Small Fruits of Jilin Province, College of Horticulture, Jilin Agricultural University, Changchun, China.

PMID: 32438500 PMCID: PMC7396441 DOI: 10.1002/2211-5463.12903

Abstract

As a small fruit rich in anthocyanins, blueberry (Vaccinium corymbosum × angustifolium) has become a focus of research in recent years for identifying genes related to anthocyanin transport and stress resistance mechanisms based on transcriptome sequencing. However, the lack of validated, stably expressed reference genes greatly limits the functional study of blueberry genes. Therefore, in this study, we selected 14 candidate reference genes from a blueberry transcriptome database and used three algorithms (geNorm, NormFinder and BestKeeper) to evaluate the expression stability of these genes in various organs at different fruit developmental stages under five abiotic stress conditions. EF1α, EIF and TBP were observed to be the most stable and were thus chosen as reference genes for quantitative real-time PCR. Measurement of the relative expression of VcMATE1 (European Nucleotide Archive accession number KF875433) in blueberry further verified the reliability of these reference genes, which may have great utility for determining the accuracy of gene expression analyses in future research on blueberry.

© 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

Keywords: RT-qPCR; abiotic stress; blueberry (Vaccinium corymbosum × angustifolium); reference gene

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