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Acta Morphol Acad Sci Hung. 1978;26(1):43-7.

A simple method for the determination of section thickness with high accuracy.

Acta morphologica Academiae Scientiarum Hungaricae

Z Fülöp, I Lakos, A M Arefjeva

PMID: 369302

Abstract

Using the partial carbon-coating of histological sections their thickness was determined by a relatively simple and accurate method. Calculations were based on the instrumental recording of the distance of focus change between the upper and lower section-surfaces. Determination of tissue refractive index was not required. Results compared favourably with those of more complicated techniques.

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